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Tissue-specific expression of an oat 12S seed glubulin gene in developing tobacco seeds: differential mRNA and protein accumulation

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Abstract

We studied the expression of the oat globulin gene asglo5 in developing transgenic tobacco seeds. The asglo5 gene promoter directed transcription in the endosperm as well as in the provascular tissue, the presumptive root tip and the shoot apical meristem of the embryo as revealed by GUS reporter gene constructs and in situ hybridization. However, immunological tissue printing detected the oat protein exclusively in the tobacco endosperm, suggesting that extensive post-transcriptional regulatory processes influence the expression of the monocot transgene in the dicot host.

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Author notes

  1. Roland Schubert

    Present address: GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Neuherberg Institut für Biochemische Pflanzenpathologie, Ingolstädter Landstrasse 1, PF 1129, D-85764, Oberschleissheim, Germany

  2. Istvan Nagy

    Present address: Agricultural Biotechnology Center, Szent-Györgyi-Albert ut. 4, P.O. Box 170, 2101, Gödöllö, Hungary

Authors and Affiliations

  1. Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, D-06466, Gatersleben, Germany

    Roland Schubert, Reinhard Panitz, Renate Manteuffel, Istvan Nagy, Ulrich Wobus & Helmut Bäumlein

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  1. Roland Schubert
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  2. Reinhard Panitz
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  3. Renate Manteuffel
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  4. Istvan Nagy
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  5. Ulrich Wobus
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  6. Helmut Bäumlein
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Schubert, R., Panitz, R., Manteuffel, R. et al. Tissue-specific expression of an oat 12S seed glubulin gene in developing tobacco seeds: differential mRNA and protein accumulation. Plant Mol Biol 26, 203–210 (1994). https://doi.org/10.1007/BF00039532

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  • DOI: https://doi.org/10.1007/BF00039532

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