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Identification and characterization of maize pathogenesis-related proteins. Four maize PR proteins are chitinases

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Abstract

Eight pathogenesis-related proteins extractable at pH 2.8 were found to accumulate in maize leaves after mercuric chloride treatment or brome mosaic virus infection. These proteins were called PRm (pathogenesis-related maize) proteins. Seven PRm proteins were purified to homogeneity by preparative polyacrylamide gel electrophoresis and their amino acid compositions determined. Estimated molecular weights in SDS-containing gels were: PRm 1 14.2 kDa; Prm 2 16.5 kDa; PRm 3 and PRm 4 25 kDa; PRm 6b 30.5 kDa; PRm 6a 32 kDa; PRm 7 34.5 kDa. Antisera raised against either PRm 3 or PRm 4 reacted specifically each with PRm 3 or PRm 4. Antisera raised against PRm 6b reacted with PRm 6b as well as with PRm 6a and antisera against PRm 7 reacted with PRm 7 and PRm 5. Tobacco anti-PR 1b antisera reacted with maize PRm 2.

Chitinase (poly[1,4-(N-acetyl-β-D-glucosamide)]glycanhydrolase, EC 3.2.1.14) activity was found for PRm 3, PRm 4, PRm 5, and PRm 7.

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Authors and Affiliations

  1. Institut de Biologie Moléculaire des Plantes, 12 rue du Général Zimmer, 67000, Strasbourg, France

    William Nasser, Marc de Tapia, Serge Kauffmann, Shideh Montasser-Kouhsari & Gérard Burkard

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  1. William Nasser
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  2. Marc de Tapia
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  3. Serge Kauffmann
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  4. Shideh Montasser-Kouhsari
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  5. Gérard Burkard
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Nasser, W., de Tapia, M., Kauffmann, S. et al. Identification and characterization of maize pathogenesis-related proteins. Four maize PR proteins are chitinases. Plant Mol Biol 11, 529–538 (1988). https://doi.org/10.1007/BF00039033

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