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Stomata of apple leaves cultured in vitro

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Abstract

Examination by scanning electron microscopy showed abaxial stomata on in vitro cultured apple (Malus pumila Mill.) leaves. With leaf ontogeny, most of these stomata appeared to lose their regulatory ability while developing wide vestibules of up to 20 μm in diameter. It is proposed that these deformed stomata may be a possible cause for the excessive transpirational water loss and consequent dehydration associated with transferring plants regenerated in vitro from culture.

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References

  1. Abbott AJ, Belcher AR (1986) Potato tuber formation in vitro. In: Withers LA, Alderson PG (Eds) Plant Tissue Culture and its Agricultural Applications (pp 113–122) Butterworths, London

    Google Scholar 

  2. Blanke MM (1987) Comparative SEM study of stomata and surface morphology in apple. Angewandte Botanik 61: 433–438

    Google Scholar 

  3. Blanke MM, Leyhe A (1988) Stomatal and cuticular transpiration of the cap and berry of grape. J Plant Physiol 132: 250–253

    Google Scholar 

  4. Brainerd KE, Fuchigami LH (1981) Acclimatization of aseptically cultured apple plants to low relative humidity. J Amer Soc Hort Sci 106: 515–518

    Google Scholar 

  5. Brainerd KE, Fuchigami LH (1982) Stomatal functioning of in vitro and greenhouse apple leaves in darkness, mannitol, ABA and CO2. J Exp Bot 33: 388–392

    Google Scholar 

  6. Constantine DR (1982) Developmental Responses in vitro and Micro-vegetative Propagation of Woody Plants. Ph.D. Thesis, Long Ashton Research Station, University of Bristol, UK

  7. Fabri A, Sutter E, Dunston SK (1986) Anatomical changes in persistent leaves of tissue-cultured strawberry plants after removal from culture. Scientia Hort 28: 331–337

    Google Scholar 

  8. Fuchigami LH, Cheng TY, Soeldner A (1981) Abaxial transpiration and water loss in aseptically cultured plum. J Amer Soc Hort Sci 106: 519–522

    Google Scholar 

  9. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15: 473–497

    Google Scholar 

  10. Wetzstein HY, Sommer HE (1983) Scanning electron microscopy of in vitro cultured Liquidambar styraciflua plantlets during acclimatization. J Amer Soc Hort Sci 108: 475–480

    Google Scholar 

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Authors and Affiliations

  1. Institut für Obstbau, Auf den Hügel 6, D-5300, Bonn, FRG

    Michael M. Blanke & Ann R. Belcher

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  1. Michael M. Blanke
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  2. Ann R. Belcher
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Blanke, M.M., Belcher, A.R. Stomata of apple leaves cultured in vitro. Plant Cell Tiss Organ Cult 19, 85–89 (1989). https://doi.org/10.1007/BF00037780

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  • DOI: https://doi.org/10.1007/BF00037780

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