Abstract
Protoplasts of two ‘leaky’ nitrate reductase deficient and thus nitrate auxotrophic (NAR) mutants of tomato and their wild types, were fused with protoplasts of monoploid potato. In all four combinations hybrid calli grew more vigorously than parental calli and this somatic hybrid vigour as such provided a useful enrichment for somatic hybrids. Selection against nitrate auxotrophy further increased the efficiency of the enrichment, particularly if a molybdenum cofactor mutation was used as the basis for the selection. It is concluded that the nitrate auxotrophy of these NAR mutants is sufficiently expressed at the level of the cell, to allow its use in somatic hybridization experiments with potato.
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Schoenmakers, H.C.H., Nobel, E.M. & Koornneef, M. Use of leaky nitrate reductase-deficient mutants of tomato (Lycopersicon esculentum Mill.) for selection of somatic hybrid cell lines with wild type potato (Solanum tuberosum L.). Plant Cell Tiss Organ Cult 31, 151–154 (1992). https://doi.org/10.1007/BF00037699
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DOI: https://doi.org/10.1007/BF00037699