Abstract
Callus cultures were induced from leaves of a tomato plant infected with tomato yellow leaf curl virus (TYLCV) and analyzed for viral DNA presence during successive subcultures. No TYLCV DNA was detected in calli sampled after eight months of culture. Considerable differences in the presence of TYLCV DNA were found within sectors of a callus culture and between different callus cultures, throughout the entire eight months period. Infected calli which were cultured at sub-optimal temperature (15°C) retained the viral DNA longer than at 25 °C. The results suggested that TYLCV disappearance during callus culture was due to a disruption of some of the cell-to-cell connections, resulting in islands of infected cells in the midst of uninfected tissue and/or to the competition between the rate of cell division and that of viral DNA replication.
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Abbreviations
- BA:
-
benzyladenine
- CMV:
-
cucumber mosaic virus
- NAA:
-
naphthaleneacetic acid
- TMV:
-
tobacco mosaic virus
- TYLCV:
-
tomato yellow leaf curl virus
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Pelah, D., Altman, A. & Czosnek, H. Tomato yellow leaf curl virus DNA in callus cultures derived from infected tomato leaves. Plant Cell Tiss Organ Cult 39, 37–42 (1994). https://doi.org/10.1007/BF00037589
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DOI: https://doi.org/10.1007/BF00037589