Abstract
Leaf and stem explants of Trillium grandiflorum and T. erectum produced mini-rhizomes (MRs) in vitro which gave rise to shoots and roots. The apical portion of the stem and the basal portion of the leaves were the most effective explants from these tissues, while stem tissue was more responsive than leaf tissue. The best response with both species was observed on half-strength MS basal medium supplemented with cytokinin and auxin. T. erectum was more responsive than T. grandiflorum overall, and in some cases produced MRs in the absence of growth regulators. Culture at 21°C appeared to stimulate growth from T. grandiflorum tissues, compared with controls at 27°C, whereas the outgrowth of shoots from MRs was inhibited in both species at 21°C. In vitro production of MRs could provide a more rapid, alternative propagation method for these species than traditional methods.
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Pence, V.C., Soukup, V.G. Factors affecting the initiation of mini-rhizomes from Trillium erectum and T. grandiflorum tissues in vitro . Plant Cell Tiss Organ Cult 35, 229–235 (1993). https://doi.org/10.1007/BF00037275
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DOI: https://doi.org/10.1007/BF00037275