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Polar lipid composition of chloroplast thylakoids isolated from leaves grown under different lighting conditions

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Abstract

The polar acyl lipid composition was determined for samples of chloroplast thylakoids isolated from Pisum sativum plants grown at light intensities of 50 and 300 μE·m-2·s-1 and from Aesculus hippocastanum leaves taken from shade or sun environments. Lighting conditions had no major effect on lipid class composition except for a small increase in the amount of monogalactosyldiacylglycerol relative to other lipids in low compared with high light and shade compared with sun conditions. The thylakoids from low light and shade environments also had, relative to those from high light and sun conditions, a substantial decrease in the level of trans-hexadecenoic acid in phosphatidyglycerol. In parallel with this there were lower lipid to chlorophyll ratios, higher overall fatty acid unsaturation, lower chlorophyll a to b ratios and increased relative levels of light harvesting chlorophyll a/b polypeptides as expected for an increase in the degree of thylakoid appression. With this in mind, our results on lipid class composition and content of trans-hexadecenoic acid are discussed in the context of the lateral distribution of lipids within the plane of membrane.

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Abbreviations

DGDG:

digalactosyldiacylglycerol

EDTA:

ethylenediaminetetraacetic acid

HEPES:

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

LHC:

light harvesting chlorophyll a/b

MGDG:

monogalactosyldiacylglycerol

MPL:

minor phospholipids

PS1:

photosystem one

PS2:

photosystem two

SDS:

sodium dodecyl sulphate

SL:

sulphoquinovosyldiacylglycerol

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Authors and Affiliations

  1. AFRC Photosynthesis Research Group, Department of Pure and Applied Biology, Imperial College of Science and Technology, SW7 2BB, London, UK

    D. J. Chapman, J. De Felice & J. Barber

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  1. D. J. Chapman
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  2. J. De Felice
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  3. J. Barber
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Chapman, D.J., De Felice, J. & Barber, J. Polar lipid composition of chloroplast thylakoids isolated from leaves grown under different lighting conditions. Photosynth Res 8, 257–265 (1986). https://doi.org/10.1007/BF00037133

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  • DOI: https://doi.org/10.1007/BF00037133

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