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‘Phytoantibodies’: a general vector for the expression of immunoglobulin domains in transgenic plants

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Abstract

Sequences encoding the immunoglobulin heavy-chain variable (VH) domains were engineered in a new general purpose vector to transform plants via Agrobacterium. The expression of an isolated VH domain (IVD) after introduction into the plant genome has been monitored by northern, western and immuno-histochemical analysis. Immunoblotting showed that the polypeptide was stably expressed and accounted for up to 1% of the soluble protein fraction. It is therefore proposed that single immunoglobulin domains of suitable specificity expressed in plants may constitute an effective system to inhibit the activity of molecules involved in plant pathology or plant development.

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Authors and Affiliations

  1. Divisione Ingegneria Genetica C.P.2400, ENEA Dipartimento Ricera e Sviluppo Agroindustrali, 00100, Roma A.D., Italy

    Eugenio Benvenuto, Raffaela Tavazza, Giorgio Ancora & Patrizia Galeffi

  2. Laboratorio de Fisiología Vegetal, Facultad de Biología, c/ Jesús Arias de Velasco, 33005, Oviedo, Spain

    Ricardo J. Ordàs

  3. CNR Istituto di Neurobiologia, Viale Marx 15, 00156, Italy

    Silvia Biocca & Antonino Cattaneo

Authors
  1. Eugenio Benvenuto
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  2. Ricardo J. Ordàs
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  3. Raffaela Tavazza
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  4. Giorgio Ancora
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  5. Silvia Biocca
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  6. Antonino Cattaneo
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  7. Patrizia Galeffi
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Benvenuto, E., Ordàs, R.J., Tavazza, R. et al. ‘Phytoantibodies’: a general vector for the expression of immunoglobulin domains in transgenic plants. Plant Mol Biol 17, 865–874 (1991). https://doi.org/10.1007/BF00037067

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  • DOI: https://doi.org/10.1007/BF00037067

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