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Molecular cloning of two novel rice cDNA sequences encoding putative calcium-dependent protein kinases

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Abstract

We have isolated, from a cDNA library constructed from rice coleoptiles, two sequences, OSCPK2 and OSCPK11, that encode for putative calcium-dependent protein kinase (CDPK) proteins. OSCPK2 and OSCPK11 cDNAs are related to SPK, another gene encoding a rice CDPK that is specifically expressed in developing seeds [20]. OSCPK2 and OSCPK11-predicted protein sequences are 533 and 542 amino acids (aa) long with a corresponding molecular mass of 59436 and 61079 Da respectively. Within their polypeptide chain, they all contain those conserved features that define a plant CDPK; kinase catalytic sequences are linked to a calmodulin-like regulatory domain through a junction region. The calmodulin-like regulatory domain of the predicted OSCPK2 protein contains 4 EF-hand calcium-binding sites while OSCPK11 has conserved just one canonical EF-hand motif. In addition, OSCPK2-and OSCPK11-predicted proteins contain, at their N-terminal region preceding the catalytic domain, a stretch of 80 or 74 residues highly rich in hydrophilic amino acids. Comparison of the NH2-terminal sequence of all three rice CDPKs so far identified (OSCPK2, OSCPK11 and SPK) indicates the presence of a conserved MGxxC(S/Q)xxT motif that may define a consensus signal for N-myristoylation. OSCPK2 and OSCPK11 proteins are both encoded by a single-copy gene and their polyadenylated transcripts are 2.4 and 3.5 kb long respectively. OSCPK2 and OSCPK11 mRNAs are equally abundant in rice roots and coleoptiles. A 12 h white light treatment of the coleoptiles reduces the amount of OSCPK2 mRNA with only a slight effect on the level of OSCPK11 transcript. With anoxic treatments, OSCPK2 mRNA level declined significantly and promptly while the amount of OSCPK11 transcript remained constant.

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References

  1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA., Struhl K: Short protocols in molecular biology; 2nd ed., pp. 5/12–5/23. Green Publishing Associates/John Wiley, New York (1992).

    Google Scholar 

  2. Battey NH: Calcium-activated protein kinase from soluble and membrane fractions of maize coleoptiles. Biochem Biophys Res Comm 170: 17–22 (1990).

    Google Scholar 

  3. Binder BM, Harper JF, Sussman MR: Characterization of an Arabidopsis calmodulin-like domain protein kinase purified from Escherichia coli using an affinity sandwich tecnique. Biochemistry 33: 2033–2041 (1994).

    Google Scholar 

  4. Bogre L, Olah Z, Duduts D: Ca2+-dependent protein kinase from alfalfa (Medicago varia): partial purification and autophosphorylation. Plant Sci 58: 135–144 (1988).

    Google Scholar 

  5. Breviario D, Giani S, DiVietri P, Coraggio I: Auxin and growth regulation of rice coleoptile segments. Plant Physiol 98: 488–495 (1992).

    Google Scholar 

  6. Budde RJA, Randall DD: Light as a signal influencing the phosphorylation status of plant proteins. Plant Physiol 94: 1501–1504 (1990).

    Google Scholar 

  7. Bustos MM, Guiltinan MJ, Cyr RJ, Ahdoot D, Fosket DE: Light regulation of β-tubulin gene expression during internode development in soybean (Glycine max L. Merr.). Plant Physiol 91: 1157–1161 (1989).

    Google Scholar 

  8. Chory J: Out of darkness: mutants reveal pathways controlling light-regulated development in plants. Trends Genet 9: 167–172 (1993).

    Google Scholar 

  9. Estruch JJ, Kadwell S, Merlin E, Crossland L: Cloning and characterization of a maize pollen-specific calcium-dependent calmodulin-independent protein kinase. Proc Natl Acad Sci USA 91: 8837–8841 (1994).

    Google Scholar 

  10. Feng XH, Kung S: Diversity of the protein kinase gene family in rice. FEBS Lett 282: 98–102 (1991).

    Google Scholar 

  11. Hank SK: Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family. Proc Natl Acad Sci USA 84: 388–392 (1987).

    Google Scholar 

  12. Hank SK, Quinn AM, Hunter T: The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241: 42–52 (1988).

    Google Scholar 

  13. Harmon AC, Yoo BC, McCaffery C: Pseudosubstrate Inhibition of CDPK, a protein kinase with a calmodulin-like domain. Biochemistry 33: 7278–7287 (1994).

    Google Scholar 

  14. Harper JF, Sussman MR, Schaller GE, Putnan-Evans C, Charbonneau H, Harmon AC: A calcium-dependent protein kinase with a regulatory domain similar to calmodulin. Science 252 951–954 (1991).

    Google Scholar 

  15. Harper JF, Binder BM, Sussman MR: Calcium and lipid regulation of an Arabidopsis protein kinase expressed in Escherichia coli. Biochemistry 32: 3282–3290 (1993).

    Google Scholar 

  16. Harper JF, Huang JF, Lloyd SJ: genetic identification of an autoinhibitor in CDPK, a protein kinase with a calmodulin-like domain. Biochemistry 33: 7267–7277 (1994).

    Google Scholar 

  17. Hepler PK, Wayne RO: Calcium and plant development. Annu Rev Plant Physiol 36: 397–439 (1985).

    Google Scholar 

  18. Hunter T: A thousand and one protein kinases. Cell 50: 823–829 (1987).

    Google Scholar 

  19. Joshi CP: Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis. Nucl Acids Res 15: 9627–9640 (1987).

    Google Scholar 

  20. Kawasaki T, Hayashida N, Baba T, Shinozaki K, Shimada H: The gene encoding a calcium-dependent protein kinase located near the sbe 1 gene encoding starch branching enzyme I is specifically expressed in developing rice seeds. Gene 129: 183–189 (1993).

    Google Scholar 

  21. Komatsu S, Hirano H: Protein kinase activity and protein phosphorylation in rice (Oryza sativa L) leaf. Plant Sci 94: 127–137 (1993).

    Google Scholar 

  22. Lawton MA, Yamamoto RT, Hanks SK, Lamb CJ: Molecular cloning of plant transcripts encoding protein kinase homologs. Proc Natl Acad Sci USA 86: 3140–3144 (1989).

    Google Scholar 

  23. Li H, Dauwalder M, Roux SJ: partial purification and characterization of a Ca2+-dependent protein kinase from pea nuclei. Plant Physiol 96: 720–727 (1991).

    Google Scholar 

  24. Ma H: Protein phosphorylation in plants. Trends Genet 9: 228–230 (1993).

    Google Scholar 

  25. Mendu N, Silflow CD: Elevated levels of tubulin transcripts accompany the GA3-induced elongation of oat internode segments. Plant Cell Physiol 34: 973–983 (1993).

    Google Scholar 

  26. Morello L, Gianì S, Coraggio I, Breviario D: Rice membranes contain a calcium-dependent protein kinase activity with biochemical features of animal protein kinase C. Biochem Biophys Res Commun 197 55–61 (1993).

    Google Scholar 

  27. Morello L, Gianì S, Breviario D: The influence of anaerobiosis on membrane-associated rice (O. sativa L.) protein kinase activities. J Plant Physiol 144: 500–504 (1994).

    Google Scholar 

  28. Putnam-Evans CL, Harmon AC, Palevitz BA, Fechheimer M, Cormier MJ: Calcium-dependent protein kinase is localized with F-actin in plant cells. Cell Motil Cytoskeleton 12: 12–22 (1989).

    Google Scholar 

  29. Putnam-Evans CL, Harmon AC, Cormier MJ: Purification and characterization of a novel calcium-dependent protein kinase from soybean. Biochemistry 29: 2488–2495 (1990).

    Google Scholar 

  30. Ranjeva R, Boudet AM: Phosphorylation of proteins in plants: regulatory effects and potential involvement in stimulus/response coupling. Annu Rev Plant Physiol 38: 73–93 (1987).

    Google Scholar 

  31. Ranjeva R, Thuleau P, Schroeder JI: Signal transduction and calcium channels in higher plants. Curr Opin Biotechnol 4: 172–176 (1993).

    Google Scholar 

  32. Reggiani R, Hochnoeppler A, Bertani A: Polyamines and anaerobic elongation of rice coleoptile. Plant Cell Physiol 30: 893–898 (1989).

    Google Scholar 

  33. Roberts DM, Harmon AC: Calcium-modulated proteins: target of intracellular calcium signals in higher plants. Annu Rev Plant Physiol 43: 375–414 (1992).

    Google Scholar 

  34. Russell Johnson D, Bhatnagar RS, Knoll LJ, Gordon JI: Genetic and biochemical studies of protein N-Myristoylation. Annu Rev Biochem 63: 869–914 (1994).

    Google Scholar 

  35. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW: Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81: 8014–8018 (1984).

    Google Scholar 

  36. Schaller GE, Sussman MR: Phosphorylation of the plasma membrane H+-ATPase of oat roots by a calcium-stimulated protein kinase. Planta 173: 509–518 (1988).

    Google Scholar 

  37. Schaller GE, Harmon AC, Sussman MR: Characterization of a calcium-and lipid-dependent protein kinase associated with the plasma membrane of oat. Biochemistry 31: 1721–1727 (1992).

    Google Scholar 

  38. Scott JD, Glaccum MB, Zoller MJ, Uhler MD, Helfman DM, McKnight GS, Krebs EG: The molecular cloning of a type II regulatory subunit of the cAMP-dependent protein kinase from rat skeletal muscle and mouse brain. Proc Natl Acad Sci USA 84: 5192–5196 (1987).

    Google Scholar 

  39. Suen KL, Choi Jh: Isolation and sequence analysis of a cDNA clone for a carrot calcium-dependent protein kinase: homology to calcium/calmodulin dependent protein kinases and to calmodulin. Plant Mol Biol 17: 581–590 (1991).

    Google Scholar 

  40. Subbaiah CC, Zhang J, Sachs MM: Involvement of intracellular calcium in anaerobic gene expression and survival of maize seedlings. Plant Physiol 105: 369–376 (1994).

    Google Scholar 

  41. Towler DA, Adams SP, Eubanks SR, Towery DS, Jackson-Machelski E, Glaser L, Gordon JI: Myristoyl CoA: protein N-myristoyltransferase activities from rat liver and yeast possess overlapping yet distinct peptide substrate specifities. J Biol Chem 263: 1784–1790 (1988).

    Google Scholar 

  42. Trewavas A, Gilroy S: Signal transduction in plant cells. Trends Genet 7: 356–361 (1991).

    Google Scholar 

  43. Tufty RM, Kretsinger RH: Troponin and parvalbumin calcium binding regions predicted in myosin light chain and T4 lysozyme. Science 187: 167–169 (1975).

    Google Scholar 

  44. Uhler MD, Chrivia JC, McKnight GS: Evidence for a second isoform of the catalytic subunit of cAMP-dependent protein kinase. J Biol Chem 261: 15360–15363 (1986).

    Google Scholar 

  45. Urao T, Katagiri T, Mizoguchi T, Yamaguchi-Shinozaki K, Hayashida N, Shinozaki K: Two genes that encode Ca2+-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thaliana. Mol Gen Genet 244: 331–340 (1994).

    Google Scholar 

  46. Weaver CD, Crombie B, Stacey G, Roberts DM: Calcium-dependent phosphorylation of symbiosome membrane proteins from nitrogen-fixing soybean nodules. Plant Physiol 95: 222–227 (1991).

    Google Scholar 

  47. Zhao Y, Kappes B, Franklin RM: Gene structure and expression of an unusual protein kinase from Plasmodium falciparum homologous at its carboxyl terminus with the EF hand calcium-binding proteins. J Biol Chem 268: 4347–4354 (1993).

    Google Scholar 

  48. Zhao Y, Pokutta S, Maurer P, Lindt M, Franklin RM, Kappes B: Calcium-binding properties of a calcium dependent protein kinase from Plasmodium falciparum and the significance of individual calcium-binding sites for kinase activation. Biochemistry 33: 3714–3721 (1994).

    Google Scholar 

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  1. Istituto Biosintesi Vegetali, C.N.R., Via Bassini 15, 20133, Milan, Italy

    Diego Breviario, Laura Morello & Silvia Gianì

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  1. Diego Breviario
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  2. Laura Morello
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  3. Silvia Gianì
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Breviario, D., Morello, L. & Gianì, S. Molecular cloning of two novel rice cDNA sequences encoding putative calcium-dependent protein kinases. Plant Mol Biol 27, 953–967 (1995). https://doi.org/10.1007/BF00037023

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  • DOI: https://doi.org/10.1007/BF00037023

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