Summary
Pollen grains of diploid and tetraploid Italian, perennial and hybrid ryegrass (Lolium spp.) germinated on 20% sucrose solution with 10 ppm each of CaCl2, H3BO3 and EDTA. Pollen tubes emerged within 2–3 minutes after sowing and completed full growth within 1 1/2 hours. The tube elongation showed the expected linear growth rate. The tubes of the haploid pollen grains grew faster (3.9 μm/minute) than that of the diploid (3.3 μm/minute). Diploid pollen grains germinated better on 25% than on 20% sucrose solution. Up to 25% of the diploid pollen produced two tubes.
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References
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Ahloowalia, B.S. Germination in vitro of ryegrass pollen grains. Euphytica 22, 575–581 (1973). https://doi.org/10.1007/BF00036658
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DOI: https://doi.org/10.1007/BF00036658