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Preparation of monoclonal antibodies against planarian organs and the effect of fixatives

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Abstract

Monoclonal antibodies were obtained against all part of the body of the planarian Dugesia japonica japonica except tthe nerve cord, though parts of the enteric canal were also non reactive. The effect of a variety of fixatives (acetone, Bouin, formalin) used on planarian tissues prior to screening with antibodies, was assessed.

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Authors and Affiliations

  1. Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu, 376, Gunma, Japan

    Takao Shinozawa, Hajime Kawarada & Kazuhisa Takezaki

  2. Department of Pharmacology, Faculty of Medicine, Gunma University, Maebashi, 371, Gunma

    Hideaki Tanaka

  3. Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, 371, Gunma

    Kinji Inoue

Authors
  1. Takao Shinozawa
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  2. Hajime Kawarada
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  3. Kazuhisa Takezaki
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  4. Hideaki Tanaka
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  5. Kinji Inoue
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Shinozawa, T., Kawarada, H., Takezaki, K. et al. Preparation of monoclonal antibodies against planarian organs and the effect of fixatives. Hydrobiologia 305, 255–257 (1995). https://doi.org/10.1007/BF00036398

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  • DOI: https://doi.org/10.1007/BF00036398

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