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Temperature dependent changes in absorption and fluorescence properties of the cyanobacterium Anacystis nidulans

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Abstract

Temperature dependent changes in absorbance and fluorescence of chlorophyll a (Chl a) were analyzed in membrane fragments and in a Chl-protein complex reconstituted with lipids isolated from the cyanobacterium Anacystis nidulans. Absorbance versus temperature curves measured at 656 nm showed an inflection point at 23–24°C and at 14–16°C in the membrane fragments prepared from A. nidulans cells, grown at 39° and 25°C, respectively. Temperature-induced absorbance changes measured at 680 and 696 nm did not show clear break points. The presence of lipids was essential in order to see a clear maximum in the fluorescence versus temperature curve of Chl a in a Chl-protein complex. It is suggested that a specific form of Chl a may be associated with lipids in the thylakoid membranes and that this form of Chl a may be responsible for temperature-induced absorbance and fluorescence yield changes in this cyanobacterium.

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Abbreviations

Chl:

chlorophyll

DCMU:

3-(3′, 4′-dichlorophenyl)-1, 1-dimethylurea

SDS:

sodium dodecyl sulphate

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Author notes

  1. Satoshi Hoshina

    Present address: Department of Biology, Faculty of Science, Kanazawa University, Macunouchi, 920, Kanazawa, Japan

  2. Prasanna Mohanty & David C. Fork

    Present address: School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India

Authors and Affiliations

  1. Department of Plant Biology, Carnegio Institution of Washington, 290 Panama Street, 94305, Stanford, CA, USA

    Satoshi Hoshina, Prasanna Mohanty & David C. Fork

Authors
  1. Satoshi Hoshina
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  2. Prasanna Mohanty
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  3. David C. Fork
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Additional information

DPB-CIW No. 802.

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Hoshina, S., Mohanty, P. & Fork, D.C. Temperature dependent changes in absorption and fluorescence properties of the cyanobacterium Anacystis nidulans . Photosynth Res 5, 347–360 (1984). https://doi.org/10.1007/BF00034979

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  • DOI: https://doi.org/10.1007/BF00034979

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