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Multiple shoot regeneration from root organ cultures of Populus alba x P. grandidentata

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Abstract

Excised roots of various ages from ‘Crandon’ and ‘Hansen’ clones of Populus alba x P. grandidentata were tested for their regeneration capacity. Sixty-day-old excised roots that contained root tips were found to be most suitable. The highest number of shoots (an average of 111 shoots/root segment with ‘Crandon’ and 98 with ‘Hansen’) was obtained by adding 22μM and 14μM zeatin to the medium, respectively. The two clones of hybrid poplar responded similarly to growth regulator treatments; however, the number of shoots produced was greater from the root organs derived from ‘Crandon’ clones. Regenerated shoots were rooted in basal Woody Plant Medium without any growth regulators. Successful transplantation into soil and growth was achieved with all plants.

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Authors and Affiliations

  1. Department of Forestry, Iowa State University, 50011, Ames, IA, USA

    Sung Ho Son & Richard B. Hall

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  1. Sung Ho Son
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  2. Richard B. Hall
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Son, S.H., Hall, R.B. Multiple shoot regeneration from root organ cultures of Populus alba x P. grandidentata . Plant Cell Tiss Organ Cult 20, 53–57 (1990). https://doi.org/10.1007/BF00034757

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  • DOI: https://doi.org/10.1007/BF00034757

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