Abstract
A procedure for the development of alfalfa (Medicago falcata L.) somatic embryos to the torpedo stage in air-lift vessels is described. Embryos were initiated from chopped leaf explants and were formed by direct somatic embryogensis. The system produced a high number of torpedo stage embryos. The effect of various inoculation densities on embryo development was studied. A procedure for the development and maturation of embryos in aerated liquid media was established. The rate of conversion of the torpedo stage embryos formed in the vessels was 83%.
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Abbreviations
- ABA:
-
abscisic acid
- B5:
-
Gamborgs B5 medium (Gamborg et al. 1968)
- COT:
-
cotyledon embryo state
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- FW:
-
fresh weight
- ID:
-
internal diameter
- MS:
-
Murashige and Skoog medium (Murashige & Skoog 1962)
- PEG:
-
polyethylene glycol
- POLY:
-
polyembryos
- VVM:
-
volume of gas/volume of bioreactor
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Kuklin, A.I., Denchev, P.D., Atanassov, A.I. et al. Alfalfa embryo production in airlift vessels via direct somatic embryogenesis. Plant Cell Tiss Organ Cult 38, 19–23 (1994). https://doi.org/10.1007/BF00034438
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DOI: https://doi.org/10.1007/BF00034438