Abstract
Plants were regenerated from the in vitro cultured explants of primary leaves of cowpea (Vigna unguiculata L. Walp). Primary leaves, including the intact petiole, were excised from three-day-old seedlings and cultured on Gamborg's B5 basal medium containing 8×10−7 M 2,4,5-trichlorophenoxyacetic acid, 1×10−2 M L-glutamine and 1×10−4 M adenine sulfate. Callus formed at the petiole end. Prolific shoot regeneration occurred when this callus was transferred to B5 basal medium containing 5×10−6 M 6-benzyl-aminopurine (BAP). Regenerated shoots rooted in growth-regulator-free B5 basal medium and were established in soil.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- IAA:
-
indole-3-acetic acid
- NAA:
-
1-napthalene acetic acid
- 2,4,5-T:
-
2,4,5-trichloro-phenoxyacetic acid
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Muthukumar, B., Mariamma, M. & Gnanam, A. Regeneration of plants from primary leaves of cowpea. Plant Cell Tiss Organ Cult 42, 153–155 (1995). https://doi.org/10.1007/BF00034232
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DOI: https://doi.org/10.1007/BF00034232