Abstract
Two year old, transformed root cultures of Catharanthus roseus accumulate ajmalicine and catharanthine (0.57 and 0.36 mg g-1 DW, or 7.0 and 3.0 mg l-1, respectively). Changes in the concentration of the medium components, as well as the addition of hydrolytic enzymes and biotic elicitors, were used as strategies to increase these alkaloid yields. Regarding the components of the medium, the results obtained, when sucrose was raised from 3 to 4.5%, are noteworthy. The nitrogen source induced differential responses in the individual alkaloid yields. No net change in the alkaloid content was observed either with changes in the concentration of vitamins or macro-and micronutrients. Though the root culture only shows a limited response to elicitors, Aspergillus treatment and the use of macerozyme increased the accumulation of ajmalicine selectively, while the addition of methyl jasmonate increased the yield of both alkaloids.
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Abbreviations
- MeJa:
-
methyl jasmonate
- mU:
-
milliunits
References
Brillanceau M-H, David C & Tempé J (1989) Genetic transformation of Catharanthus roseus G. Don by Agrobacterium rhizogenes. Plant Cell Rep. 8: 63–66
Brooks CJW, Watson DG & Freer IM (1986) Elicitation of capsidiol accumulation in suspended callus cultures of Capsicum annuum. Phytochemistry 25: 1089–1092
Chappell J & Nable R (1987) Induction of sesquiterpenoid biosynthesis in tobacco cell suspension cultures by fungal elicitor. Plant Physiol. 85: 469–473
Ciau-Uitz R, Miranda-Ham ML, Coello-Coello J, Chí B, Pacheco LM & Loyola-Vargas VM (1994) Indole alkaloid production by transformed and non-transformed root cultures of Catharanthus roseus. In vitro Cell. Develop. Biol. Plant, in press
Collinge M & Brodelius P (1989) Dynamics of benzophenanthridine alkaloid production in suspension cultures of Eschscholtzia californica after treatment with a yeast elicitor. Phytochemistry 28: 1101–1104
DiCosmo F & Misawa M (1985) Eliciting secondary metabolism in plant cell cultures. Trends Biotechnol. 3: 318–322
Dubois M, Gilles KA, Hamilton JK, Rebers PA & Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356
Eilert U, Constabel F & Kurz WGW (1986) Elicitor-stimulation of monoterpene indole alkaloid formation in suspension cultures of Catharanthus roseus. J. Plant Physiol. 126: 11–22
Farmer EE & Ryan CA (1990) Interplant communication: airbone methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proc. Natl. Acad. Sci. USA 87: 7713–7716
Flores HE, Pickard JJ & Signs M (1988) Elicitation of polyacetylene production in hairy root cultures of Asteraceae. Plant Physiol. 86: 108s Abstract
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158
Godoy-Hernández G & Loyola-Vargas VM (1991) Effect of fungal homogenate, enzyme inhibitors and osmotic stress on alkaloid content of Catharanthus roseus cell suspension cultures. Plant Cell Rep. 10: 537–540
Gundlach H, Müller MJ, Kutchan TM & Zenk MH (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc. Natl. Acad. Sci. USA 89: 2389–2393
Jung KH, Kwak SS, Kim SW, Lee H, Choi CY & Liu JR (1992) Improvement of catharanthine productivity in hairy root cultures of C. roseus by using monosaccharides as carbon source. Biotechnol. Lett. 14: 695–700
Knobloch K-H & Berlin J (1980) Influence of medium composition on the formation of secondary compounds in cell suspension cultures of Catharanthus roseus (L.) G. Don. Z. Naturforsch. 35c: 551–556
Kombrink E & Hahlbrock K (1986) Responses of cultured parsley cells to elicitors from phytopathogenic fungi. Timing and dose dependency of elicitor-induced reactions. Plant Physiol. 81: 216–221
Kurz WGW, Constabel F, Eilert U & Tyler RT (1987) Elicitor treatment: a method for metabolite production by plant cell cultures in vitro. In: Breimer DD & Speiser P (Ed) Topics in Pharmaceutical Sciences 1987, (pp 283–290). Elsevier Science Publishers
Monforte-González M, Ayora-Talavera T, Maldonado-Mendoza IE & Loyola-Vargas VM (1992) Quantitative analysis of serpentine and ajmalicine in plant tissue of Catharanthus roseus and hyoscyamine and scopolamine in root tissues of Datura stramonium by densitometry in thin layer chromatography. Phytochem. Anal. 3: 117–121
Morris P (1986) Regulation of product synthesis in cell cultures of Catharanthus roseus. II. Comparison of production media. Planta Med. 52: 121–126
Mukundan U & Hjortso MA (1990a) Thiophene accumulation in hairy roots of Tagetes patula in response to fungal elicitors. Biotechnol. Lett. 12: 609–614
Mukundan U & Hjortso MA (1990b) Effect of fungal elicitor on thiophene production in hairy root cultures of Tagetes patula. Appl. Microbiol. Biotechnol. 33: 145–147
Nef C, Rio B & Chrestin H (1991) Induction of catharanthine synthesis and stimulation of major indole alkaloids production by Catharanthus roseus cells under non-growth-altering treatment with Pythium vexans extracts. Plant Cell Rep. 10: 26–29
Parr AJ, Peerless ACJ, Hamill JD, Walton NJ, Robins RJ & Rhodes MJC (1988) Alkaloid production by transformed root cultures of Catharanthus roseus. Plant Cell Rep. 7: 309–312
Smith JI, Quesnel A, Smart NJ, Misawa M & Kurz WGW (1987) The development of a single-stage growth and indole alkaloid production medium for Catharanthus roseus (L.) G. Don suspension cultures. Enzyme Microbiol. Technol. 9: 466–469
Stöckigt J (1980) The biosynthesis of heteryohimbine-type alkaloids. In: Phillipson JD & Zenk MH (Ed) Indole and biogenetically related alkaloids, (pp 113–141). Academic Press, London
Threlfall DR & Whitehead IM (1988) The use of metal ions to induce the formation of secondary products in plant tissue culture. In: Robins RJ & Rhodes MJC (Ed) Manipulating Secondary Metabolism in Culture, (pp 51–56). Cambridge University Press, Cambridge
Toivonen L, Balsevich J & Kurz WGW (1989) Indole alkaloid production by hairy root cultures of Catharanthus roseus. Plant Cell Tissue Organ Cult. 18: 79–93
Toivonen L, Ojala M & Kauppinen V (1990) Indole alkaloid production by hairy root cultures of Catharanthus roseus: growth kinetics and fermentation. Biotechnol. Lett. 12: 519–524
Toivonen L, Ojala M & Kauppinen V (1991) Studies on the optimization of growth and indole alkaloid production by hairy root cultures of Catharanthus roseus. Biotechnol. Bioeng. 37: 673–680
Vázquez-Flota F, Coello J & Loyola-Vargas VM (1992) Growth kinetics and alkaloid production in hairy root cultures of Catharanthus roseus. Plant Physiol. 99: 49s (Abstract)
Vögeli U & Chappell J (1988) Induction of sesquiterpene cyclase and suppression of squalene synthetase activities in plant cell cultures treated with fungal elicitor. Plant Physiol. 88: 1291–1296
Zenk MH, El-Shagi H, Arens H, Stöckigt J, Weiler EW & Deus B (1977) Formation of the indole alkaloids serpentine and ajmalicine in cell suspension cultures of Catharanthus roseus. In: Barz W, Reinhard E & Zenk MH (Ed) Plant Tissue Culture and its Bio-technological Application, (pp 27–43). Springer-Verlag, Berlin
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Vázquez-Flota, F., Moreno-Valenzuela, O., Miranda-Ham, M.L. et al. Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy root cultures. Plant Cell Tiss Organ Cult 38, 273–279 (1994). https://doi.org/10.1007/BF00033887
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DOI: https://doi.org/10.1007/BF00033887