Abstract
We have studied the effect of various in-vitro conditions on dormancy of bulblets generated on scale explants of Lilium speciosum Thunb. cv. ‘Rubrum’ nr. 10. The bulblets were harvested after 11 weeks of culture. Dormancy was measured by determining the percent emergence in soil of viable, non-cold-treated bulblets. A study of the physical conditions showed that temperature had a strong effect on the induction of dormancy (15°C induced hardly any dormancy; 25°C induced a high level of dormancy), whereas short or long day and light or dark had no effect. Of the medium components, a low concentration of sucrose (1 gl−1 or less) or a high concentration of gibberellic acid (1 mg 1−1) reduced the level of dormancy. Application of various concentrations of abscisic acid, 6-benzylaminopurine, α-naphthaleneacetic acid, indole-3-acetic acid, 2,3,5-triiodobenzoic acid or a Murashige and Skoog macro- and microelement mixture did not affect the dormancy status.
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Abbreviations
- ABA:
-
abscisic acid
- BAP:
-
6-benzylaminopurine
- GA3 :
-
gibberellic acid
- IAA:
-
indole-3-acetic acid
- MS:
-
Murashige & Skoog macro- and microelements
- NAA:
-
α-naphthalene-acetic acid
- TIBA:
-
2,3,5-triiodobenzoic acid
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Aguettaz, P., Paffen, A., Delvallée, I. et al. The development of dormancy in bulblets of Lilium speciosum generated in vitro. Plant Cell Tiss Organ Cult 22, 167–172 (1990). https://doi.org/10.1007/BF00033631
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DOI: https://doi.org/10.1007/BF00033631