Abstract
Different Gram-positive and Gram-negative bacteria (Staphylococcus xylosus, S. aureus, S. cohnii, Bacillus sp., Corynebacterium sp., Pseudomonas vesicularis) were isolated from homogenized shoot tips of Drosera rotundifolia, Spatiphyllum sp., Syngonium cv. White butterfly, Nephrolepis exaltata cv. Teddy Junior. Growth inhibition of selected bacterial strains was examined using 28 different single antibiotics and 7 antibiotic mixtures. It was found that with the two mixtures Imipenem/Ampicillin and Imipenem/Penicillin G at concentrations of 5 mg l−1 each, bacterial growth inhibition was most effective. Because of the lack of toxic effects on in vitro plants of 7 species it was proposed that these antibiotic mixtures can be applied advantageously to inhibit bacterial growth in tissue culture.
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References
Attafuah A & Bradbury JF (1989) Pseudomonas antimicrobica, a new species strongly antagonistic to plant pathogens. J. Appl. Bact. 67: 567–573
Boxus PH & Terzi J-M (1987) Big losses due to bacterial contamination can be avoided in mass propagation scheme. Acta Hort. 212: 91–93
Cornu D & Michel MF (1987) Bacterial contaminants in shoot cultures of Prunus avium L., choice and phytotoxicity of antibiotics. Acta Hort. 212: 83–86
George EF & Sherrington PD (1984) Plant Propagation by Tissue Culture (pp 88–93). Exegetics Ltd, England
Holt JG (1986) Bergey's Manual of Systematic Bacteriology, Vol 2. Williams & Wilkins, Baltimore, London, Los Angeles, Sydney
Leifert C, Waites WM & Nicholas JR (1989) Bacterial contaminants in micropropagated plant cultures. J. Appl. Bact. 67: 353–361
Leifert C, Waites WM, Nicholas JR & Keetley JWW (1990) Yeast contaminants of micropropagated plant cultures. J. Appl. Bact. 69: 471–476
Lloyd G & McCown B (1981) Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia, by use of shoot tip culture. Int. Plant Prop. Soc. Proc. 30: 421–427
Mathias PJ, Alderson PG & Leaky RRB (1987) Bacterial contamination in hardwood cultures. Acta Hort. 212: 43–48
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Okkels FT & Pederson MG (1983) The toxicity to plant tissue and to Agrobacterium tumefaciens of some antibiotics. Acta Hort. 225: 199–207
Podwyszynska M & Hempel M (1987) Identification and elimination of ‘slowly growing’ bacteria from micropropagated Gerbera. Acta Hort. 212: 112
Trick I & Lingens F (1985) Aerobic spore-forming bacteria as detrimental infectants in plant tissue cultures. Appl. Microbiol. Biotechnol. 21: 245–249
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Kneifel, W., Leonhardt, W. Testing of different antibiotics against Gram-positive and Gram-negative bacteria isolated from plant tissue culture. Plant Cell Tiss Organ Cult 29, 139–144 (1992). https://doi.org/10.1007/BF00033619
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DOI: https://doi.org/10.1007/BF00033619