Abstract
Cells of a 2-year-old suspension culture of rice (Oryza sativa L.), grown under 1.5% NaCl stress for 3 months, gave rise to plants through embryogenesis in different saline conditions. The high regeneration potential (59.6%) on salt-free medium decreased rapidly with increasing concentration of salt in the regeneration medium. At 1.25% NaCl, healthy shoots were developed in 14.9% of the cultures. Under 1.5% salt stress, embryo formation and embryo germination (6.1%) was observed but further development into plants was inhibited. Cells not pretreated with salt produced plants at a low frequency (2.6–4.2%) both in salt-free and low saline condition (0.75–1% NaCl). Cells pretreated for 3 months with 0.75% salt did not give rise to plants on all tested media. Plants regenerated from the salt-stressed cultures were transferred to soil and grew to maturity in a greenhouse.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BA:
-
6-benzyladenine
- CH:
-
casein hydrolysate
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphthaleneacetic acid
References
Abe T & Futsuhara Y (1968) Plant regeneration from suspension cultures of rice Oryza sativa L. Japan J. Breed. 36: 1–6
Akbar M & Ponnamperuma FN (1982) Saline soils of South and Southeast Asia as potential rice lands. In: Rice Research Strategies for the Future (pp 265–281). Int. Rice Res. Inst., P.O. Box 933, Manila, Philippines
Akbar M & Yabuno T (1974) Breeding for saline-resistant varieties of rice. II. Comparative performance of some rice varieties to salinity during early developmental stages. Japan J. Breed. 24: 176–181
Akbar M, Khush GS & Hillerislambers D (1986) Genetics of salt tolerance in rice. In: Rice Genetics (pp 399–409). Proceeding of the International Rice Genetics Symposium. 27–31 May, 1985. Int. Rice Res. Inst., P.O. Box 933. Manila, Philippines
Binh DQ & Heszky LE (1990) Restoration of the regeneration potential of long-term cell culture in rice (Oryza sativa L.) by salt pretreatment. J. Plant Physiol. 136: 336–340
Binh DQ, Heszky LE, Gyulai G, Kiss E & Csillag A (1989) Plant regeneration from callus of Puccinellia distans (L.) Parl. Plant Cell Tiss. Org. Cult. 18: 195–200
Carter DL (1975) Problems of salinity in agriculture. In: Poljakoff-Mayber A & Gale I (Eds) Plants in Saline Environments (pp 25–35). Springer-Verlag, Berlin-Heidelberg-New York
Chu CC (1978) The N6 medium and its application to another culture of cereal crops. In: Proceeding of the Symposium on Plant Tissue Culture (pp 43–50) Peking, Science Press, Beijing
Croughan TP, Stavarek SJ & Rains DW (1978) Selection of NaCl tolerant line of cultured alfalfa cells. Crop. Sci. 18: 959–963
Epstein E, Norlyn JD, Rush DW, Kingsbury RW, Kelley DB, Cunningham GA & Wrona AF (1980) Saline culture of crops: a genetic approach. Science 210: 399–404
Galiba G & Yamada Y (1988) A novel method for increasing the frequency of somatic embryogenesis in wheat tissue culture by NaCl and KCl supplementation. Plant Cell Rep. 7: 55–58
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158
Göbel E, Ozias-Akins P & Lörz H (1986) Cell and protoplast culture of rice. In: Withers LA & Alderson PG (Eds) Plant Tissue Culture and Its Agricultural Applications (pp 359–365). Butterworths, Kent
Heszky LE, Li SN & Horvath ZS (1986) Rice tissue culture and application to breeding. II. Factors affecting the plant regeneration during subculture of diploid and haploid callus. Cereal Res. Comm. 14: 289–296
Heszky LE, Li SN, Kiss E, Simon-Kiss I, Lökös K & Binh DQ (1991) In vitro studies on rice in Hungary. In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry, Vol 14 (pp 619–641). (Rice), Springer Verlag, Berlin-Heidelberg-New York
Li SN & Heszky LE (1986) Testing of salt (NaCl) tolerance and regeneration in callus culture (n, 2n) of rice. In: Horn W, Jensen JC, Odenbach W & Schieder O (Eds) Genetic Manipulation in Plant Breeding (pp 617–619). Walter de Gruyter and Co, Berlin-New York
Müller AJ & Grafe R (1978) Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol. Gen. Genet. 161: 67–76
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nabors MW & Dykes TA (1984) Tissue culture of cereal cultivars with increased salt, drought, and acid tolerance. In: Biotechnology in International Agricultural Research (pp 121–138). International rice Research Institute, Manila, Philippines
Nabors MW, Gibbs SE, Berstein CS & Meis ME (1980) NaCl tolerant tobacco plants from cultured cells. Z. Pflanzenphysiol. 97: 13–17
Nabors MW, Heyser TA, Dykes TA & DeMott KJ (1983) Long duration, high frequency plant regeneration from cereal tissue cultures. Planta 97: 13–17
Rains DW, Croughan TP & Stavarek SJ (1980) Selection of salt tolerant plants using tissue culture. In: Rains DW, Valentine RC & Hollaender A (Eds) Genetic Engineering of Osmoregulation: Impact on Plant Productivity for Food, Chemicals and Energy (pp 279–292). Plenum Press, New York
Reddy PJ & Vaidyanath K (1986) In vitro characterization of salt stress effects and the selection of salt tolerant plants in rice (Oryza sativa L.). Theor. Appl. Genet. 71: 757–760
Vajrabhaya M, Thanapaisal T & Vajrabhaya T (1989) Development of salt tolerant lines of KDML and LPT rice cultivars through tissue culture. Plant Cell Rep. 8: 411–414
Vasil IK (1983) Toward the development of a single cell system for grasses. In: Cell and Tissue Culture Techniques for Cereal Crop Improvement (pp 131–144). Science Press, Beijing
Yeo AR & Flowers TJ (1983) Varietal differences in the toxicity of sodium ions in rice leaves. Physiol. Plant. 59: 189–195
Yoshida S, Ogawa M, Suenaga K & Ye HC (1983) Induction and selection of salt-tolerant mutant rices by tissue culture — Recent progress at IRRI. In: Cell and Tissue Culture Techniques for Cereal Crop Improvement (pp 237–254). Science Press, Beijing
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Binh, D.Q., Heszky, L.E., Gyulai, G. et al. Plant regeneration of NaCl-pretreated cells from long-term suspension culture of rice (Oryza sativa L.) in high saline conditions. Plant Cell Tiss Organ Cult 29, 75–82 (1992). https://doi.org/10.1007/BF00033611
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00033611