Abstract
In order to save manual labour in plant tissue propagation, we tried adding liquid media to established, exhausted cultures, instead of transplanting the tissues to a fresh medium. This has been investigated for the elongation and for root induction stages with Cordyline, Philodendron, Magnolia and Spathiphyllum. The salt concentration and the application time are important factors, determining the success of this method. Charcoal added to the liquid stage IIIa medium eliminated the residual effects of BA applied during stage II.
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Maene, L., Debergh, P. Liquid medium additions to established tissue cultures to improve elongation and rooting in vivo. Plant Cell Tiss Organ Cult 5, 23–33 (1985). https://doi.org/10.1007/BF00033566
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DOI: https://doi.org/10.1007/BF00033566