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Microanalysis of plant mitochondrial protein synthesis products:

Detection of variant polypeptides associated with cytoplasmic male sterility

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Summary

A mitochondrial fraction obtained from 0.5 g of leaves was purified on a 0.75 ml Percoll gradient and used for an in vitro mitochondrial protein synthesis assay in the presence of [35S] methionine. A set of 15 to 20 labeled polypeptides were revealed by autoradiography after sodium dodecylsulfate polyacrylamide gel electrophoresis. This could be applied at an early growth stage by using a few leaves from individual seedlings. It revealed the presence of variant mitochondrially translated polypeptides in green leaves of cytoplasmic male sterile lines from various cultivated plants of large economic importance: maize, wheat, sugar beet, tobacco and faba bean. This non-destructive microanalysis is thus of general use and opens new possibilities for rapid and large mass screening of mitochondrial parameters such as male sterility.

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Author notes

  1. Marc Boutry

    Present address: Laboratoire d'Etude de l'Hérédité Cytoplasmique, Université Catholique de Louvain, Place Croix du Sud, 1, 1348, Louvain-la-Neuve, Belgium

Authors and Affiliations

  1. Laboratoire d'Etude de l'Hérédité Cytoplasmique, Université Catholique de Louvain, Place Croix du Sud, 1, 1348, Louvain-la-Neuve, Belgium

    Maryse Charbonnier & Michel Briquet

  2. The Rockefeller University, 1230 York Avenue, 10021-6399, New York, NY, U.S.A.

    Marc Boutry & Anne-Marie Faber

Authors
  1. Marc Boutry
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  2. Anne-Marie Faber
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  3. Maryse Charbonnier
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  4. Michel Briquet
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Boutry, M., Faber, AM., Charbonnier, M. et al. Microanalysis of plant mitochondrial protein synthesis products:. Plant Mol Biol 3, 445–452 (1984). https://doi.org/10.1007/BF00033392

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  • DOI: https://doi.org/10.1007/BF00033392

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