Summary
Shallot and wakegi were collected in the main islands of Indonesia, and in Japan, Korea, Taiwan, Malaysia, Thailand and Bangladesh. Five isozyme resolutions, phosphoglucomutase (PGM), glutamate oxaloacetate (GOT), glutamate dehydrogenase (GDH), esterase (EST) and peroxidase (POX) were employed for demonstrating inter-and intraspecific differences. A dendrogram separated 189 collected accessions into 25 types of wakegi onion and 18 types of shallot. All accessions of Japan, Korea and Taiwan were determined to be wakegi onion, whereas those of Bangladesh, Malaysia and Thailand were shallot. Twenty-six out of 165 Indonesian accessions indicated wakegi onion distribution in Sumatra, West Java province and in Sulawesi Island. This confirmed that there is mixed-cultivation of the two Allium species with no distinction made between them. Japan and Indonesia had respectively 12 and eight unique types of wakegi onion, while Korea had only one type. West Java showed the most various type of wakegi onion, whereas East Java had many types of shallot. Shallots collected from Bangladesh were distinetly different from those of South East Asian types.
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References
Arus, P., 1983. Genetic purity of commercial seed lots. In: S.D.Tanksley & T.J.Orton (Eds.) Isozymes in Plant Genetics and Breeding. Part A, pp. 415–424. Elsevier Science Publishers B.V., Amsterdam.
Central Bureau of Statistic, Indonesia, 1992. Nanpudi Jaya. Jakarta. pp. 204–207.
Cochran, E.D., 1942. Cytogenic studies of species hybrid A. fistulosum and A. ascalonicum and its back cross progenies. Ph D Thesis Univ Calif Davis. pp. 1–14.
Currah, L. & F.J.Proctor, 1990. Onion in tropical regions. Chapt 2. The genetic base and the cultivars grown in the tropics. Natural Resources Institute. Central Evenue 35: 31–42.
Goodman, M.M. & C.W.Stuber, 1983. Maize. In: S.D.Tanksley & T.J.Orton (Eds.) Isozymes in Plant Genetics and Breeding. Part B, pp. 1–34. Elsevier Science Publishers B.V., Amsterdam.
Hadacova, V., E.Klozova, E.Hadac, V.Turkova & K.Pitterova, 1981. Comparison of esterase isozyme patterns in seeds of some Allium sp. and in cultivars of A. cepa L. Biol Plant (Praha) 23: 174–181.
Haishima, M., J.Kato & H.Ikehashi, 1993. Isozyme polymorphism in native varieties of Japanese bunching onion (Allium fistulosum L.). Japan J Breed 43: 537–547.
Hanelt, P., 1990. Taxonomy, evolution, and history. In: H.D.Rabinowitch & J.L.Brewster (Eds.) Onions and Allied Crops, Vol. I. Botany, Physiology, and Genetics, pp. 1–26. CRC Press, Boca Raton, Florida.
Inden, H. & T.Asahira, 1990. Japanese bunching onion (Allium fistulosum L.). In: J.L.Brewster & H.D.Rabinowitch (Eds.) Onions and Allied Crops, Vol. III. Biochemistry, Food Science, and Minor Crops, pp. 159–178. CRC Press, Boca Raton, Florida.
Mancini, R., C.DePace, G.T.Scarascia Mugnozza, V.Delre & D.Vittori, 1989. Isozyme gene markers in Vicia faba L. Theor Appl Genet 77: 657–667.
Nauen, W. & T.Hartmann, 1980. Glutamate dehydrogenase from Pisum. Planta 148: 7–16.
Okubo, H. & K.Fujieda, 1989. Intraspecific differentiation and isozyme patterns in Allium wakegi Araki. J Japan Soc Hort Sci 58: 401–406 (in Japanese with English summary).
Peffley, E.B. & L.Currah, 1988. The chromosomal locations of enzyme coding genes ADH-1 and PGM-1 in A. fistulosum L., Theor Appl Genet 75: 945–949.
Peffley, E.B. & C.Orozco-Castillo, 1987. Polymorphism of isozymes within plant introductions of Allium cepa L. and A. fistulosum L. HortScience 22: 956–957.
Peffley, E.B., J.N.Corgan, K.E.Horak & S.D.Tanksley, 1985. Electrophoretic analysis of Allium alien addition lines. Theor Appl Genet 71: 176–184.
Pryor, A.J., 1974. Notes and comments. Allelic glutamic dehydrogenase isozymes in maize. A single hybrid isozyme in heterozygotes? Heredity 32: 397–401.
Shigyo, M., Y.Tashiro & S.Miyazaki, 1983. Genetic analyses of isozymes in Allium. 4. Glutamate dehydrogenase isozymes in A. fistulosum, A. ascalonicum, A. cepa, A. x wakegi and wild species in section cepa. J Japan Soc Hort Sci 62 (Suppl 2): 192–193 (in Japanese).
Sneath, P.H.A. & R.R.Sokal, 1973. Numerical taxonomy. WH Freeman, San Francisco. pp. 100–308.
Sukhorzhevskaya, T.B., 1980. Investigation of genetic control over glutamate dehydrogenase in maize (Zea mays L.). Genetica 16: 914–917.
Tashiro, Y., 1980. Cytogenetic studies on the origin of Allium wakegi Araki. I. Meiosis in A. x wakegi and the interspecific hybrid between A. fistulosum L. and A. ascalonicum L. Agr Bull, Saga Univ 49: 47–57.
Tashiro, Y., 1981. Cytogenetic studies on the origin of Allium wakegi Araki. II. Fertility and meiotic behavior of the tetraploid A. x wakegi and the amphidiploid hybrid between A. ascalonicum L. and A. fistulosum L. Agr Bull, Saga Univ 50: 85–91.
Tashiro, Y., S.Miyazaki, K.Kanazawa & H.Hashimoto, 1983. Cytogenic studies on the origin of Allium wakegi Araki. III. Restoration of the fertility of A. x wakegi by doubling the chromosome complement. Bull Fac Agric, Saga Univ 55: 125–129.
Tashiro, Y., 1984. Genome analysis of Allium wakegi Araki. J Japan Soc Hort Sci 52: 399–407.
Tashiro, Y., K.Amakawa, T.Mizutani, M.A.Quadir & S.Miyazaki, 1990. Genetic analysis of isozymes in Allium. 1. Aspartate transaminase isozymes in A. fistulosum, A. cepa, A. ascalonicum and A. x wakegi. J Japan Soc Hort Sci 59 (Suppl 1): 232–233 (in Japanese).
Wendel,J.F., 1983. Electrophoretic analysis of genetic variation in wild and cultivated Camellia japonica L. Ph D Thesis, University of North Carolina, Chapel Hill, North Carolina.
Wendel, J.F. & N.F.Weeden, 1989. Visualization and interpretation of plant isozymes. In: D.E.Soltis & P.S.Soltis (Eds.) Isozymes in Plant Biology, pp. 5–45. Dioscorides Press, Porland, Oregon.
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Arifin, N.S., Okudo, H. Geographical distribution of allozyme patterns in shallot (Allium cepa var. ascalonicum Backer) and wakegi onion (A. × wakegi Araki). Euphytica 91, 305–313 (1996). https://doi.org/10.1007/BF00033092
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DOI: https://doi.org/10.1007/BF00033092