Abstract
Leaf water potential was decreased by withholding irrigation to provide three levels of stress described as mild ({ie69-1}) moderate ({ie69-2}) and severe ({ie69-3}). The specific activity of NADP linked glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, aldolase, phosphogluco-isomerase and RuBP carboxylase decreased under mild stress, but the activity of phosphoglucomutase showed an increase whilst ribulose-5-phosphate kinase was least affected. With further decrease in water potential, the activity of NADP linked glyceraldehyde-3-phosphate dehydrogenase and aldolase showed a decrease, whereas, the activities of fructose-1,6-bisphosphatase, phosphoglycerate kinase, phosphogulcomutase and RuBP carboxylase increased. Net CO2 fixation decreased sharply with stress, whereas, respiration and photorespiration increased in moderate stress, but decreased under severe stress. Stomatal resistance also increased with decrease in water potential. It seems that in vitro enzyme activities of PCR cycle are not responsible for decreased photosynthesis in pigeonpea under short term water stress.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ackerson RC, Krieg DR, Harming CL and Chang H (1977) Effect of plant water status on stomatal activity, photosynthesis and nitrate reductase activity of field grown cotton. Crop Sci 17: 81–84
Baier D and Latzko E (1975) Properties and regulation of C-1-fructose-1, 6-diphosphatase from spinach chloroplasts. Biochim Biophys Acta 396: 141–148
Berkowitz GA and Gibbs M (1983) Reduced osmotic potential inhibition of photosynthesis. Site specific effects of osmotically induced stromal acidification. Plant Physiol 72: 1100–1109
Boyer JS (1976) Water deficits and photosynthesis. In Kozlowski TT, (ed) Water deficit and plant growth, Vol IV pp 153–190. Academic Press, New York
Dabas S and Sheoran IS (1984) Photosynthesis, respiration and stomatal conductance in two species of Brassica under water stress. Indian J Plant Physiol 27:(in press)
Hanson AD and Hitz WD (1982) Metabolic responses of mesophytes to plant water deficits. Ann Rev Plant Physiol 33: 163–203
Hsiao TC (1973) Plant responses to water stress. Ann Rev Plant Physiol 24:519–570
Huffaker RC, Radin T, Klein kopf GE and Cox EL (1970) Effects of mild water stress on enzymes of nitrate assimilation and of the carboxylative phase of photosynthesis in barley. Crop Sci 10:471–474
Jones HG (1973) Moderate-term water stresses and associated changes in some photosynthetic parameter in cotton. New Phytol 72:1095–1105
Jones HG and Rawson HM (1979) Influence of rate of development of leaf water deficits upon photosynthesis, leaf conductance, water use efficiency and osmotic potential in sorghum. Physiol Plant 45:103–111
Keck RW and Boyer JS (1974) Chloroplast response to low leaf water potentials III. Differing inhibition of electron transport and photophosphorylation. Plant Physiol 53:474–479
Kreig DR (1983) Photosynthetic activity during stress. Agric water Manage 7:247–263
Latzko E and Gibbs M (1968) Distribution and activity of enzymes of the reductive pentose phosphate cycle in spinach leaves and in chloroplasts isolated by different methods. Z Pflanzenphysiol 59:184–194
Lawlor DW and Fock H (1975) Photosynthesis and photorespiratory CO2 evolution of water stressed sunflower leaves. Planta 126:247–258
Lawlor DW and Fock H (1977) Water stress induced changes in the amounts of some photosynthetic assimilation products and respiratory metabolites of sunflower leaves. J Exp Bot 28:329–337
Lee KC, Campbell RW and Paulsen GM (1974) Effects of drought stress and succinic acid 2, 2-dimethylhydrazide treatment on water relations and photosynthesis in pea seedlings. Crop Sci 14:279–282
Lilley MCR and Walker DA (1974) An improved spectrophotometric assay for ribulosebisphosphate carboxylase. Biochim Biophys Acta 358:226–229
Lowry OH, Rosebrough NJ, Farr AL and Randall RJ (1951) Protein measurement with Folin-phenol reagent. J Biol Chem 193:265–275
Luthra YP, Sheoran IS and Randhir Singh (1983a) Ontogenic interactions between photosynthesis and symbiotic nitrogen fixation in pigeonpea. Ann Appl Biol 103:549–556
Luthra YP, Sheoran IS and Randhir Singh (1983b) Ontogenic changes in the level of ureides and enzymes of their metabolism in various plant parts of pigeonpea. J Exp Bot 34:1358–1370
O'Toole JC, Ozburn JL and Wallace DH (1977) Photosynthetic response to water stress in Phaseolus vulgaris. Physiol Plant 40:111–114
Racker E (1957) The reductive penose phosphate cycle. I Phosphoribulokinase and ribulose diphosphate carboxylase. Arch Biochem Biophys 69: 300–310
Sharkey TD and Badger MR (1982) Effects of water stress on photosynthetic electron transport, photophosphorylation and metabolite levels of Xanthium strumarium mesophyll cells. Plants 156:199–206
Shearman LL, Eastin JD, Sullivan CV and Kinbacher EJ (1972) Carbon dioxide exchange in water stressed sorghum. Crop Sci 12:406–409
Sheoran IS, Luthra YP, Kuhad MS and Randhir Singh (1981) Effect of water stress on some enzymes on nitrogen metabolism in pigeonpea. Phytochemistry 20:2675–2677
Stewart GR and Lee JA (1972) Desiccation injury in mosses. II The effect of moisture stress on enzyme levels. New Phytol 71:461–466
Sutton BG (1975) Glycolysis in CAM plant. Aust J Plant Physiol 2:389–402
Wu R and Racker E (1959) Regulatory mechanisms in carbohydrate metabolism. III limiting factors in glycolysis of ascites tumor cells. J Biol Chem 234:1029–1035
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Singal, H.R., Sheoran, I.S. & Singh, R. Effect of water stress on photosynthesis and in vitro activities of the PCR cycle enzymes in pigeonpea (Cajanus cajan L.). Photosynth Res 7, 69–76 (1985). https://doi.org/10.1007/BF00032923
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00032923