Abstract
A method for reducing hyperhydricity of lettuce shoots regenerated from culture on liquid and gelled media is described. In the case of liquid medium, hyperhydricity could be decreased by 30% by using a filter paper raft or a polyester screen raft to prevent immersion of inoculum in the culture medium. In the case of gelled medium, replacing agar with the water-absorbent resin SuperSorb could successfully prevent hyperhydricity without depressing organogenesis.
This is a preview of subscription content, log in via an institution to check access.
References
Chin CK, Kong Y & Pedersen H (1988) Culture of droplets containing asparagus cells and protoplasts on polypropylene membrane. Plant Cell Tiss. Org. Cult. 5: 59–65
Gilmour DM, Davey MR, Cocking EC & Pental D (1987) Culture of low numbers of forage legume protoplasts in membrane chambers. J. Plant Physiol. 126: 457–465
Leshem B (1983) The carnation succulent plantlet-a stable teratological growth. Ann. Bot. 52: 873–876
Pasqualetto PL (1990) Vitrification in plant tissue culture. In: Rodriguez R, Tamus RS & Durzan DJ (Eds) Plant aging: Basic and Applied Approaches (pp 133–137). Plenum Press, New York
Schenk RU & Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous platn cell culture. Can. J. Bot. 50: 199–204
Teng WL, Liu YJ & Soong TS (1992) Rapid regeneration of lettuce from suspension culture. HortScience 27: 1030–1032
Ziv M (1991) Vitrification: morphological and physiological disorders of in vitro plants. In: Debergh PC & Zimmerman RH (Eds), Micropropagtion Technology and Application (pp 45–49). Kluwer Academic Publishers, Dordrecht
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Teng, WL., Liu, YJ. Restricting hyperhydricity of lettuce using screen raft and water absorbent resin. Plant Cell Tiss Organ Cult 34, 311–314 (1993). https://doi.org/10.1007/BF00029723
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00029723