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Somatic embryogenesis from immature zygotic embryos of oil palm

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Abstract

Immature zygotic embryos at different developmental stages were used for callus induction and regeneration studies. Immature embryos excised from fruits 77, 91, 100, 114, 128, 140 and 193 days after pollination and mature embryos were cultured on modified Y3 medium containing 500 mgl−1 cysteine, 0.5% (w/v) PVP-40, 500 μM 2,4-d and 0.3% (w/v) charcoal. Compact embryogenic tissue began differentiating directly from embryo explants after 2 weeks of culture. The percentage of embryos forming compact embryogenic tissue ranged from 28.6% for 91-day-old embryos to 0% for 140-day-old and older embryos. Friable embryogenic tissue was observed in callus cultures derived from 100-day-old embryos. Although both compact and friable embryogenic tissues were successfully isolated, normal embryo and plantlet development was observed only from friable embryogenic tissue.

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Abbreviations

ABA:

abscisic acid

2,4-d :

2,4-dichlorophenoxyacetic acid

NAA:

naphthaleneacetic acid

PVP:

polyvinylpyrollidone

References

  • Ahee J, Arthuis P, Cas G, Duval Y Guenin G, Janower J, Hanower P, Lievoux D, Lioret C, Malaurie B, Pannetier C, Raillot D, Varechon C & Zuckerman L (1981) La multiplication végétative in vitro du palmier à huile per embryogénèse somatique. Oléagineaux 36: 113–115

    Google Scholar 

  • Ammar S & Benbadis A (1977) Multiplication végétative du palmierdattier (Phoenix dactylifera L.) par la culture de tissus de jeunes plantes issues de semis. C.R. Acad. Sci. Paris 284: 1789–1792

    Google Scholar 

  • Armstrong CL & Green CE (1985) Establishment and maintenance of friable, embryogenic maize callus and the involvement of l-proline. Planta 164: 207–214

    Google Scholar 

  • Eeuwens CJ (1976) Mineral requirements for growth and callus inhibition of tissue explants excised from mature coconut palms (Cocos nucifera L.) and culture in vitro. Physiol. Plant. 36: 23–28

    Google Scholar 

  • Eeuwens CJ (1978) Effect of organic nutrients and hormones on growth and development of tissue explants from coconut (Cocos nucifera L.) and date (Phoenix dactylifera L.) palms cultured in vitro. Physiol. Plant. 42: 173–178

    Google Scholar 

  • Eeuwens CJ & Blake J (1977) Culture of coconut and date palm tissue with a view to vegetative propagation. Acta Hort. 78: 277–286

    Google Scholar 

  • Green CE (1982) Somatic embryogenesis and plant regeneration from the friable callus of Zea mays In: Fujiwara A (Ed) Plant Tissue Culture (pp 107–108). Maruzen Co., Tokyo

    Google Scholar 

  • Green CE & Phillips RL (1975) Plant regeneration from tissue cultures of maize. Crop Sci. 15: 417–421

    Google Scholar 

  • Guerra PM & Handro W (1988) Somatic embryogenesis and plant regeneration in embryo cultures of Euterpe edulis Mart. (Palmae). Plant Cell Rep. 7: 550–552

    Article  Google Scholar 

  • Hanower J & Pannetier C (1982) In vitro vegetative propagation of the oil palm (Elaeis guineensis Jacq.). In: Fujiwara A (Ed) Plant Tissue Culture (pp 745–746). Maruzen Co., Tokyo

    Google Scholar 

  • Jones L H (1974) Propagation of clonal oil palms by tissue culture. Oil Palm News 17: 1–8

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Nwankwo BA & Krikorian AD (1983) Morphogenetic potential of embryo and seedling-derived callus of Elaeis guineensis Jacq. var. Pisifera Becc. Ann. Bot. 51: 65–76

    Google Scholar 

  • Pannetier C, Arthuis P & Lievoux D (1981) Néoformatin de jeunes plantes d'Elaeis guineensis à partir de cals primaires obtenus sur fragments foliaries cultivés in vitro. Oléagineux 36: 119–120

    Google Scholar 

  • Prioli L & Söndahl MR (1989) Plant regeneration and recovery of fertile plants from protoplasts of maize (Zea mays L.). BioTechnology 7: 589–594

    Article  Google Scholar 

  • Rabechault H & Martin JP (1976) Multiplication végétative du palmier à huile (Elaeis guineensis Jacq.) à l'aide de culture de tissus foliaires. C.R. Acad. Sci. Paris 283: 1735–1737

    Google Scholar 

  • Rabechault H, Ahee J & Guenin G (1970) Colonies cellulaires et formes embryoides obentues in vitro à partir de cultures d'embryons de Palmier à huile (Elaeis guineensis Jacq. var. dura Becc.). C.R. Acad. Sci. Paris 270: 3067–3070

    Google Scholar 

  • Rabechault H, Martin JP Cas S (1972) Recherches sur la culture de palmier à huile (Elaeis guineensis Jacq.) Oléagineux 27: 531–534

    Google Scholar 

  • Reuveni O & Lilien-Kipnis H (1974) Studies on the in vitro culture of date palm (Phoenix dactylifera L.) Pamphlet No. 145, Volcani Inst. Agr. Res. Israel

    Google Scholar 

  • Reynolds JF & Murashige T (1979) Asexual embryogenesis in callus cultures of palms. In Vitro 5: 383–387

    Google Scholar 

  • Sharp WR, Söndahl MR, Caldas LS & Maraffa SB (1980) The physiology of in vitro a sexual embryogenesis. Hort. Rev. 2: 268–310

    Google Scholar 

  • Shillito RD, Carswell GK, Johnson CM, Dimaio JJ & Harms CT (1989) Regeneration of fertile plants from protoplasts of elite inbred maize. BioTechnology 7: 581–588

    Article  Google Scholar 

  • Singer SR (1986) Analyzing growth in cell cultures. I. Calculating growth rates. Can. J. Bot. 64: 233–241

    Google Scholar 

  • Smith WK & Thomas JA (1973) The isolation and in vitro cultivation of cells of Elaeis guineensis. Oléagineux 28: 123–127

    Google Scholar 

  • Söndahl MR, Spahlinger DA & Sharp WR (1979) A histological study of high frequency and low frequency induction of somatic embryos in cultured leaf explants of Coffea arabica L. Z. Pflanzenphysiol. 94: 101–108

    Google Scholar 

  • Street HE (1979) Embryogenesis and chemically induced organogenesis. In: Sharp WR, Jansen PO, Paddock EF & Raghavan V (Eds) Plant Cell and Tissue Culture. Principles and Applications (pp 123–153). Ohio State University Press. Columbus

    Google Scholar 

  • Tisserat B (1979) Propagation of date palm (Phoenix dactylifera L.) J. Exp. Bot. 30: 1275–1283

    Google Scholar 

  • Turnham J & Northcote DH (1982) The use of acetyl-CoA carboxylase activity and changes in wall composition as measures of embryogenesis in tissue cultures of oil palm (Elaeis guineenis). Biochem. J. 208: 323–332

    PubMed  Google Scholar 

  • Ziv M & Halevy AH (1983) Control of oxidative browning and in vitro propagation of Strelitzia reginae. HortScience 18: 434–436

    Google Scholar 

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Author notes

  1. J. B. Teixeira

    Present address: CENARGEN, EMBRAPA, P.O. Box 02372, Brasilia, DF, Brazil

Authors and Affiliations

  1. DNA Plant Technology Corp., 2611 Branch Pike, 08077, Cinnaminson, N.J., USA

    J. B. Teixeira & M. R. Söndahl

  2. Department of Biological Sciences, Rutgers University, 07102, Newark, N.J., USA

    E. G. Kirby

Authors
  1. J. B. Teixeira
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  2. M. R. Söndahl
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  3. E. G. Kirby
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Teixeira, J.B., Söndahl, M.R. & Kirby, E.G. Somatic embryogenesis from immature zygotic embryos of oil palm. Plant Cell Tiss Organ Cult 34, 227–233 (1993). https://doi.org/10.1007/BF00029711

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  • DOI: https://doi.org/10.1007/BF00029711

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