Abstract
Accumulation of the 28 KD protein of the glutelin-(G2) fraction was followed in developing maize endosperm, using sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and peak integration of scanned gels. 28 KD glutelin-2 could already be observed from 15 days after pollination and its accumulates reached a plateau during the second half of the development period. The process of biosynthesis of 28 KD glutelin-2 and zeins occurs in a parallel way. Subcellular fractions obtained from linear sucrose gradient centrifugation of developing maize endosperms were analyzed by SDS-PAGE and immunoblotting using a serum reacting against glutelin-2 and 14 KD Z2. Glutelin-2 was found to be present in the protein bodies when subcellular fractionation was carried out without dithiothreitol (DTT). The presence of a reducing agent causes the elution of glutelin-2 from protein bodies. Immunocytochemical labelling using the protein A-colloidal gold technique in protein bodies incubated with anti-G2 IgG revealed that G2 is located mainly in the periphery of protein bodies. These results are interpreted as indicating a structural role for glutelins in protein bodies.
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References
Burr, B, Burr, FA: Zein synthesis in maize endosperm by polyribosomes attached to protein bodies. Proc Natl Acad Sci USA 73: 515–519, 1976.
Burr, FA, Burr, B: In vitro uptake and processing of prezein and other maize preproteins by maize membranes. J Cell Biol 90: 427–434, 1981.
Dierks-Ventling, C, Cosens, K: Immunochemical crossreactivity between zein, hordein and gliadin. FEBS Lett 142: 147–150, 1982.
Dierks-Ventling, C: Storage proteins in Zea mays (L): Interrelationship of Albumins, Globulins and Zeins in the opaque-2 mutation. Eur J Biochem 120: 177–182, 1981.
DiFonzo, N, Fornasari, E, Salamini, F, Soave, C: SDS-protein subunits in normal, opaque-2 and floury-2 maize endosperms. Maydica 22: 77–88, 1977.
Essen, A, Bietz, JA, Paulis, JW, Wall, JS: Fractionation of alcohol-soluble reduced corn glutelines on phosphocellulose an partial characterization of two proline-rich fractions. Cereal Chem 58: 534–537, 1981.
Essen, A, Bietz, JA, Paulis, JW, Wall, JS: Tandem repeats in the N-terminal sequence of a proline-rich protein from corn endosperm. Nature 296: 678–679, 1982.
Ginazza, E, Righetti, PG, Pioli, F, Galante, E, Soave, C: Size and charge heterogeneity of zein in normal and opaque-2 maize endosperms. Maydica 21: 1–17, 1976.
Hagen, C, Rubenstein, I: Complex organization of zein genes in maize. Gene 13: 239–249, 1980.
Laemmli, UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, Lond 227: 680–685, 1970.
Landry, J, Moureaux, T: Physicochemical properties of maize glutelins as influenced by their isolation conditions. J Agric Food Chem 29: 1205–1212, 1981.
Larkins, BA, Dalby, A: In vitro synthesis of zein like protein by maize polyribosomes. Biochem Biophys Res Commun 66: 1048–1054, 1975.
Larkins, BA, Hurkman, WJ: Synthesis and deposition of zein in protein bodies of maize endosperm. Plant Physiol 62: 256–263, 1978.
Morrisey, JH: Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem 117: 307–310, 1981.
Pedersen, K, Devereux, J, Wilson, D-R, Sheldon, E, Larkins, BA: Cloning and sequence analysis reveal structural variation related zein genes in maize. Cell 29: 1015–1026, 1982.
Pernollet, JC, Kim, S, Mossé, J: Characterization of storage proteins extracted from Avena sativa seed protein bodies. J Agric Food Chem 30: 32–36, 1982.
Renart, J, Reiser, J, Stark, GR: Transfer of proteins from gels to diazobenzylxymethyl paper and detection with antisera: a method of studying antibody specificity and antigen structure. Proc Natl Acad Sci USA 76: 3116–3120, 1979.
Roth, J, Bendayan, M, Carlemalm, E, Villiger, W, Garavito, M: The enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem 29: 663–671, 1981.
Slot, JW, Geuze, HJ: Sizing of protein A-colloidal gold probes for immunoelectron microscopy. J Cell Biol 90: 533–536, 1981.
Spena, A, Viotti, A, Pirrota, V: A homologous repetitive block structure underlies the heterogeneity of heavy and light chain zein genes. EMBO J 1: 1589–1594, 1982.
Tsai, CY: Tissue-specific zein synthesis in maize kernel. Biochem Genet 17: 1109–1119, 1979.
Vitale, A: Smaniotto, E, Longhi, T, Galante, E: Reduced soluble proteins associated with maize endosperm proteion bodies. J Exp Bot 33: 439–448, 1982.
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Ludevid, M.D., Torrent, M., Martinez-Izquierdo, J.A. et al. Subcellular localization of glutelin-2 in maize (Zea mays L.) endosperm. Plant Mol Biol 3, 227–234 (1984). https://doi.org/10.1007/BF00029658
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DOI: https://doi.org/10.1007/BF00029658