Skip to main content
SpringerLink
Log in

Characterization of an Arabidopsis thaliana gene (TMKL1) encoding a putative transmembrane protein with an unusual kinase-like domain

  • Update Section
  • Short Communication
  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

We have characterized a novel Arabidopsis gene, designated TMKL1 (for Transmembrane Kinase-Like). The encoded protein is predicted to contain an N-terminal signal peptide, an extracellular domain with seven imperfect leucine-rich repeats, a single hydrophobic transmembrane segment, and a kinase-like intracellular domain which lacks, however, several of the key conserved residues essential for catalytic activity. The TMKL1 protein thus represents a unique and functionally intriguing member of the family of plant proteins related to animal receptor kinases. Northern blot analysis indicates that the TMKL1 gene is transcriptionally active in a variety of organs, and is developmentally regulated during silique maturation. Its possible functions are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Braun T, Schofield PR, Sprengel R: Amino-terminal leucine-rich repeats in gonadotropin receptors determine hormone selectivity. EMBO J 10: 1885–1890 (1991).

    PubMed  Google Scholar 

  2. Chang C, Schaller GE, Patterson SE, Kwok SF, Meyerowitz EM, Bleecker AB: The TMK1 gene from Arabidopsis codes for a protein with structural and biochemical characteristics of a receptor protein kinase. Plant Cell 4: 1263–1271 (1992).

    Article  PubMed  Google Scholar 

  3. Devereux J, Haeberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395 (1984).

    PubMed  Google Scholar 

  4. Downing WL, Mauxion F, Fauvarque M-O, Reviron M-P, de Vienne D, Vartanian N, Giraudat J: A Brassica napus transcript encoding a protein related to the Künitz protease inhibitor family accumulates upon water stress in leaves, not in seeds. Plant J 2: 685–693 (1992).

    Article  PubMed  Google Scholar 

  5. Fuller F, Porter JG, Arfsten AE, Miller J, Schilling JW, Scarborough RM, Lewicki JA, Schenk DB: Atrial natriuretic peptide clearance receptor: complete sequence and functional expression of cDNA clones. J Biol Chem 263: 9395–9401 (1988).

    PubMed  Google Scholar 

  6. Giraudat J, Hauge BM, Valon C, Smalle J, Parcy F, Goodman HM: Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell 4: 1251–1261 (1992).

    Article  PubMed  Google Scholar 

  7. Goring DR, Rothstein SJ: The S-Locus receptor kinase gene in a self-incompatible Brassica napus line encodes a functional serine/threonine kinase. Plant Cell 4: 1273–1281 (1992).

    Article  PubMed  Google Scholar 

  8. Hanks SK, Quinn AM, Hunter T: The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241: 42–52 (1988).

    PubMed  Google Scholar 

  9. Hashimoto C, Hudson KL, Anderson KV: The Toll gene of Drosophila, required for dorsal-ventral embryonic polarity, appears to encode a transmembrane protein. Cell 52: 269–279 (1988).

    Article  PubMed  Google Scholar 

  10. Kataoka T, Broek D, Wigler M: DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase. Cell 43: 493–505 (1985).

    Article  PubMed  Google Scholar 

  11. Keith FJ, Gay N: The Drosophila membrane receptor Toll can function to promote cellular adhesion. EMBO J 9: 4299–4306 (1990).

    PubMed  Google Scholar 

  12. Knighton DR, Zheng J, Eyck LFT, Ashford VA, Xuong N-H, Taylor SS, Sowadski JM: Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science 253: 407–414 (1991).

    PubMed  Google Scholar 

  13. Knighton DR, Zheng J, Eyck LFT, Xuong N-H, Taylor SS, Sowadski JM: Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science 253: 414–420 (1991).

    PubMed  Google Scholar 

  14. Kohorn BD, Lane S, Smith TA: An Arabidopsis serine/threonine kinase homologue with an epidermal growth factor repeat selected in yeast for its specificity for a thylakoid membrane protein. Proc Natl Acad Sci USA 89: 10989–10992 (1992).

    PubMed  Google Scholar 

  15. Lopez JA, Chung DW, Fujikawa K, Hagen FS, Papayannopoulou T, Roth GJ: Cloning of the α chain of human platelet glycoprotein Ib: A transmembrane protein with homology to leucine-rich α2-glycoprotein. Proc Natl Acad Sci USA 84: 5615–5619 (1987).

    PubMed  Google Scholar 

  16. Lowe DG, Chang MS, Hellmiss R, Chen E, Singh S, Garbers DL, Goeddel DV: Human atrial natriuretic peptide receptor defines a new paradigm for second messenger signal transduction. EMBO J 8: 1377–1384 (1989).

    PubMed  Google Scholar 

  17. Lütcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA: Selection of AUG initiation codons differes in plants and animals. EMBO J 6: 43–48 (1987).

    PubMed  Google Scholar 

  18. Massagué J: Receptors for the TGF-β family. Cell 69: 1067–1070 (1992).

    Article  PubMed  Google Scholar 

  19. McFarland KC, Sprengel R, Phillips HS, Köhler M, Rosemblit N, Nikolics K, Segaloff DL, Seeburg PH: Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor family. Science 245: 494–499 (1989).

    PubMed  Google Scholar 

  20. Rothberg JM, Jacobs JR, Goodman CS, Artavanis-Tsakonas S: slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains. Genes Devel 4: 2169–2187 (1990).

    PubMed  Google Scholar 

  21. Schindler M, Meiners S, Cheresh DA: RGD-dependent linkage between plant cell wall and plasma membrane: consequences for growth. J Cell Biol 108: 1955–1965 (1989).

    Article  PubMed  Google Scholar 

  22. Schneider R, Schweiger M: A novel modular mosaic of cell adhesion motifs in the extracellular domains of the neurogenic trk and trkB tyrosine kinase receptors. Oncogene 6: 1807–1811 (1991).

    PubMed  Google Scholar 

  23. Stein JC, Howlett B, Boyes DC, Nasrallah ME, Nasrallah JB: Molecular cloning of a putative receptor kinase gene encoded at the self-incompatibility locus of Brassica oleraced. Proc Natl Acad Sci USA 88: 8816–8820 (1991).

    PubMed  Google Scholar 

  24. Suzuki N, Choe H-R, Nishida Y, Yamawaki-Kataoka Y, Ohnishi S, Tamaoki T, Kataoka T: Leucine-rich repeats and carboxyl-terminus are required for interaction of yeast adenylate cyclase with RAS proteins. Proc Natl Acad Sci USA 87: 8711–8715 (1990).

    PubMed  Google Scholar 

  25. Tobias CM, Howlett B, Nasrallah JB: An Arabidopsis thaliana gene with sequence similarity to the S-locus receptor kinase of Brassica oleracea: Sequence and expression. Plant Physiol 99: 284–290 (1992).

    Google Scholar 

  26. Trewavas A, Gilroy S: Signal transduction in plant cells. Trends Genet 7: 356–361 (1991).

    PubMed  Google Scholar 

  27. Ullrich A, Schlessinger J: Signal transduction by receptors with tyrosine kinase activity. Cell 61: 203–212 (1990).

    Article  PubMed  Google Scholar 

  28. von Heijne G: How signal sequences maintain cleavage specificity. J Mol Biol 173: 243–251 (1984).

    Article  PubMed  Google Scholar 

  29. Walker JC: Receptor-like protein kinase genes of Arabidopsis thaliana. Plant J 3: 451–456 (1993).

    Article  PubMed  Google Scholar 

  30. Walker JC, Zhang R: Relationship of a putative receptor kinase from maize to the S-locus glycoproteins of Brassica. Nature 345: 743–746 (1990).

    Article  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Jan Smalle

    Present address: Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000, Gent, Belgium

Authors and Affiliations

  1. Centre National de la Recherche Scientifique UPR 40, Institut des Sciences Végétales, 91198, Gif-sur-Yvette Cedex, France

    Christiane Valon & Jérôme Giraudat

  2. Department of Genetics, Harvard Medical School, 02114, Boston, MA, USA

    Jan Smalle & Howard M. Goodman

  3. Department of Molecular Biology, Massachusetts General Hospital, 02114, Boston, MA, USA

    Jan Smalle & Howard M. Goodman

Authors
  1. Christiane Valon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jan Smalle
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Howard M. Goodman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jérôme Giraudat
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Valon, C., Smalle, J., Goodman, H.M. et al. Characterization of an Arabidopsis thaliana gene (TMKL1) encoding a putative transmembrane protein with an unusual kinase-like domain. Plant Mol Biol 23, 415–421 (1993). https://doi.org/10.1007/BF00029017

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00029017

Key words

Search

Navigation