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Cloning and characterization of five cDNAs for genes differentially expressed during fruit development of kiwifruit (Actinidia deliciosa var.deliciosa)

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Abstract

Five cDNAs for genes differentially expressed during fruit development of kiwifruit (Actinidia deliciosa var.deliciosa cv. Hayward) were isolated from a library made from young fruit, 8–10 days after anthesis. One gene (pKIWI503) has low levels of expression in young fruit but is induced late in fruit development and during fruit ripening, and has some homology to plant metallothionein-like proteins. The other four genes are highly expressed in young fruit with reduced expression in the later stages of fruit development. pKIWI504 has strong homology to plant metallothionein-like proteins and pKIWI505 exhibits homology to the β-subunit of the mitochondrial ATP synthase gene. The two other genes (pKIWI501 and 502) encode proteins with no significant homology to other known sequences.

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References

  1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol 215: 403–410 (1990).

    Article  PubMed  Google Scholar 

  2. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K. Current Protocols in Molecular Biology. John Wiley, New York (1989).

    Google Scholar 

  3. Boedtker H, Frischauf A, Lehrach H: Isolation and translation of calvaria procollagen messenger ribonucleic acids. Biochemistry 15: 4765–4770 (1976).

    PubMed  Google Scholar 

  4. Boutry M, Chua N: A nuclear gene encoding the β-subunit of the mitochondrial ATP synthase inNicotiana plumbaginifolia. EMBO J 4: 2159–2165 (1985).

    PubMed  Google Scholar 

  5. Callahan AM, Morgens PH, Cohen RA. Isolation and initial characterization of cDNAs for mRNAs regulated during peach fruit development. J Am Soc Hort Sci 118: 531–537 (1993).

    Google Scholar 

  6. Chye M, Tan C: Isolation and nucleotide sequence of a cDNA clone encoding the β-subunit of mitochondrial ATP synthase fromHevea brasiliensis. Plant Mol Biol 18: 611–612 (1992).

    PubMed  Google Scholar 

  7. Clark CJ, Smith GS: Seasonal accumulation of mineral nutrients by kiwifruit. 2. Fruit. New Phytol 108: 399–409 (1988).

    Google Scholar 

  8. de Framond AJ: A metallothionein-like gene from maize (Zea mays): cloning and characterization. FEBS Lett 290: 103–106 (1991).

    Article  PubMed  Google Scholar 

  9. de Miranda JR, Thomas MA, Thurman DA, Tomsett AB: Metallothionein genes from the flowering plantMimulus guttatus. FEBS Lett 260: 277–280 (1990).

    Article  PubMed  Google Scholar 

  10. Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395 (1984).

    PubMed  Google Scholar 

  11. Evans IM, Gatehouse LN, Gatehouse JA, Robinson NJ, Croy RR. A gene from pea (Pisum sativum L.) with homology to metallothionein genes. FEBS Lett 262: 29–32 (1990).

    Article  PubMed  Google Scholar 

  12. Evans KM, Gatehouse JA, Lindsay WP, Shi J, Tommey AM, Robinson NJ: Expression of the pea metallothionein-like genePsMT A inEscherichia coli andArabidopsis thaliana and analysis of trace metal ion accumulation; implications forPsMT A function. Plant Mol Biol 20: 1019–1028 (1992).

    PubMed  Google Scholar 

  13. Fourney RM, Miyakoshi J, Day RS, Paterson MC: Northern blotting: efficient RNA staining and transfer. Focus 10: 5–7 (1988).

    Google Scholar 

  14. Garboczi DN, Fox AH, Gerring SL, Pedersen PL: β-subunit of rat liver mitochondrial ATP synthase: cDNA cloning, amino acid sequence, expression inEscherichia coli, and structural relationship to adenylate kinase. Biochemistry 27: 553–560 (1988).

    PubMed  Google Scholar 

  15. Gray J, Picton S, Shabbeer J, Schuch W, Grierson D: Molecular biology of fruit ripening and its manipulation with antisense genes. Plant Mol Biol 19: 69–87 (1992).

    PubMed  Google Scholar 

  16. Harvey CF, Fraser LG, Pavis SE, Considine JA: Floral biology of two species ofActinidia (Actinidiaceae). 1. The stigma, pollination, and fertilization. Bot Gaz 148: 426–432 (1987).

    Article  Google Scholar 

  17. Hopping ME: Structure and development of fruit and seeds in Chinese gooseberry (Actinidia chinensis Planch.). NZ J Bot 14: 63–68 (1976).

    Google Scholar 

  18. Kawashima I, Inokuchi Y, Chino M, Kimura M, Shimizu N: Isolation of a gene for a metallothionein-like protein from soybean. Plant Cell Physiol 32: 913–916 (1991).

    Google Scholar 

  19. King GA, Davies KM: Identification, cDNA cloning and analysis of mRNAs having altered expression in tips of harvested asoaragus spears. Plant Physiol 100: 1661–1669 (1992).

    Google Scholar 

  20. Klemsdal SS, Hughes W, Lonneborg A, Aalen RB, Olsen O: Primary structure of a novel barley gene differentially expressed in immature aleurone layers. Mol Gen Genet 228: 9–16 (1991).

    Article  PubMed  Google Scholar 

  21. Koning AJ, Tanimoto EY, Kiehne K, Rost T, Comai L: Cell-specific expression of plant histone H2A genes. Plant Cell 3: 657–665 (1991).

    Article  PubMed  Google Scholar 

  22. Martineau B, McBride KE, Houck CM: Regulation of metallocarboxypeptidase inhibitor gene expression in tomato. Mol Gen Genet 228: 281–286 (1991).

    Article  PubMed  Google Scholar 

  23. Moore KB, Oishi KK. Characterization of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity during maize seed development, germination and seedling emergence. Plant Physiol 101: 485–491 (1993).

    PubMed  Google Scholar 

  24. Narita JO, Gruissem W: Tomato hydroxymethylglutaryl-CoA reductase is required early in fruit development but not during ripening. Plant Cell 1: 181–190 (1989).

    Article  PubMed  Google Scholar 

  25. Ohta S, Kagawa Y: Human F1-ATPase: molecular cloning of cDNA for the β-subunit. J Biochem 99: 135–141 (1986).

    PubMed  Google Scholar 

  26. Okumura N, Nishizawa N, Umehara Y, Mori S: An iron deficiency-specific cDNA from barley roots having two homologous cysteine-rich MT domains. Plant Mol Biol 17: 531–533 (1991).

    PubMed  Google Scholar 

  27. Pear JR, Ridge N, Rasmussen R, Rose RE, Houck CM: Isolation and characterization of a fruit-specific cDNA and the corresponding genomic clone from tomato. Plant Mol Biol 13: 639–651 (1989).

    Article  PubMed  Google Scholar 

  28. Praekelt UM, McKee RA, Smith H: Molecular analysis of actinidin, the cysteine proteinase ofActinidia chinensis. Plant Mol Biol 10: 193–202 (1988).

    Google Scholar 

  29. Pratt HK, Reid MS: Chinese Gooseberry: Seasonal patterns in fruit growth and maturation, ripening, respiration and the role of ethylene. J Sci Food Agric 25: 747–757 (1974).

    Google Scholar 

  30. Sakamoto M, Shimada H, Jugimura T: Nucleotide sequence of a cDNA encoding a β-subunit of the mitochondrial ATPase from rice (Oryza sativa). Plant Mol Biol 20: 171–174 (1992).

    PubMed  Google Scholar 

  31. Salts Y, Wachs R, Gruissem W, Barg R: Sequence coding for a novel proline-rich protein preferentially expressed in young tomato fruit. Plant Mol Biol 17: 149–150 (1991).

    PubMed  Google Scholar 

  32. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).

    PubMed  Google Scholar 

  33. Smith GS, Buwalda JG, Clark CJ: Nutrient dynamics of a kiwifruit ecosystem. Sci Hort 37: 87–109 (1988).

    Article  Google Scholar 

  34. Snowden KC, Gardner RC. Five genes induced by aluminum in wheat (Triticum aestivum L.) roots. Plant Physiol 103: 855–861 (1993).

    Article  PubMed  Google Scholar 

  35. Tommey AM, Shi J, Lindsay WP, Urwin PE, Robinson NJ: Expression of the pea genePsMT A inE. coli: metal-binding properties of the expressed protein. FEBS Lett 292: 48–52 (1991).

    Article  PubMed  Google Scholar 

  36. Van Haaren MJ, Houck CM: A functional map of the fruit-specific promoter of the tomato 2A11 gene. Plant Mol Biol 21: 625–640 (1993).

    PubMed  Google Scholar 

  37. Varga A, Bruinsma J. Tomato. In: Monselise SP (ed) CRC handbook of Fruit Set and Development, pp. 461–480. CRC Press, Boca Raton, FL (1986).

    Google Scholar 

  38. Walton EF, de Jong TM: Growth and compositional changes in kiwifruit berries from three Californian locations. Ann Bot 66: 285–298 (1990).

    Google Scholar 

  39. Winning BM, Bathgate B, Purdue PE, Leaver CJ: Nucleotide sequence of a cDNA encoding the β-subunit of the mitochondrial ATP synthase fromZea mays. Nucl Acids Res 18: 5885 (1990).

    PubMed  Google Scholar 

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  1. Centre for Gene Technology, School of Biological Sciences, University of Auckland, Private Bag, 92019, Auckland, New Zealand

    Susan E. Ledger & Richard C. Gardner

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  1. Susan E. Ledger
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  2. Richard C. Gardner
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Ledger, S.E., Gardner, R.C. Cloning and characterization of five cDNAs for genes differentially expressed during fruit development of kiwifruit (Actinidia deliciosa var.deliciosa). Plant Mol Biol 25, 877–886 (1994). https://doi.org/10.1007/BF00028882

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  • DOI: https://doi.org/10.1007/BF00028882

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