Abstract
Carbon dioxide concentration during growth is commonly not considered to be a factor influencing the photochemical properties of plants. It was observed that fluorescence induction in Chlamydomonas reinhardii cells grown at air levels of CO2 was both qualitatively and quantitatively different from that of cells grown at 5% CO2. In the two cell types, measured at equivalent chlorophyll and irradiance levels, the fluorescence intensity and the ratio of the levels of peak fluorescence (Fp) to that of the initial fluorescence (Fo) were much lower in the air-adapted than in the 5% CO2 adapted cells. The maximum fluorescence (Fmax) in the presence of diuron was also lower for air-adapted cells. Roughly twice the light input was required for the air-adapted cells to give a fluorescence induction transient and intensity equivalent to that of the 5% CO2-adapted cells. Similar properties were observed in several other unicellular green algae and in cyanobacteria. Chlamydomonas grown under variable CO2 concentrations exhibit significant differences in photosynthetic carbon metabolism and are presumed to have altered energy requirements. The observed variation in fluorescence induction may be due to changes in the properties of the thylakoid reactions (e.g. cyclic electron flow) of Chlamydomonas cells, which may, in turn, be due to a response to the altered energy requirements.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Badger MR, Kaplan A and Berry JA (1980) Internal inorganic carbon pool of Chlamydomonas reinhardii. Evidence for a carbon dioxide concentrating mechanism. Plant Physiol 66: 407–413
Badger MR and Andrews TJ (1983) Photosynthesis and inorganic carbon usage by the marine cyanobacterium, Synechococcus sp. Plant Physiol 70: 517–523
Barber J (1976) Ionic regulation in intact chloroplasts and its effect on primary photosynthetic processes. In Barber J (ed), The Intact Chloroplast, Elsevier, Amsterdam, pp. 89–134
Björkman O (1981) Responses to different quantum flux densities. In Lange OL, Nobel PS, Osmond CB and Ziegler H (eds), Physiological Ecology I, Encyclopedia of Plant Physiology, New Series, Vol 12A, Springer, Berlin, pp. 57–107
Boardman NK (1977) Comparative photosynthesis of sun and shade plants. Ann Rev Plant Physiol 28: 355–377
Govindjee and Papageorgiou G (1971) Chlorophyll fluorescence and photosynthesis: fluorescence transients. In Giese AC (ed), Photosphysiology, Vol 6, Acad Press, New York, pp. 1–46
Govindjee and Van Rensen JJ (1978) Bicarbonate effects on electron flow in isolated broken chloroplasts. Biochim Biophys Acta 505: 183–213
Haworth P, Kyle DJ, Horton P and Arntzen CJ (1982) Chloroplast membrane protein photophosphorylation. Photochem Photobiol 36: 743–748
Horton P (1983) Control of chloroplast electron transport by phosphorylation of thylakoid proteins. FEBS Lett 153: 47–52
Horton P and Lee P (1983) Stimulation of a cyclic electron-transfer pathway around photosystem II by phosphorylation of chloroplast thylakoid proteins. FEBS Lett 162: 81–84
Kaplan A, Badger MR and Berry JA (1980) Photosynthesis and the intracellular inorganic carbon pool in the blue-green alga Anabaena variabilis: Planta 149: 219–226
Lavorel J and Etienne AL (1977) In vivo chlorophyll fluorescence. In Barber J (ed), Primary Processes of Photosynthesis, Elsevier, Amsterdam, pp. 203–268
Malkin S, Armond PA, Mooney HA and Fork DC (1981) Photosystem II photosynthetic unit sizes from fluorescence induction in leaves. Plant Physiol 67: 570–579
Melis A and Harvey CW (1981) Regulation of photosystem stoichiometry, chlorophyll a and chlorophyll b content and relation to chloroplast ultrastructure. Biochem Biophys Acta 637 138–145
Mende D (1980) Evidence for a cyclic PS-II-electron transport in vivo.Plant Sci Lett 17: 215–220
Mende D, Niemeyer H, Hecker S and Wiessner K (1978) In vivo regulation of the photosynthetic electron transport. Photosynthetica 12: 440–448
Munday JC and Govindjee (1969) Light-induced changes in the fluorescence yield of chlorophyll a in vivo III. The dip and the peak in the fluorescence transients of Chlorella pyrenoidosa. Biophysic J 9: 1–21
Papageorgiou G (1975) Chlorophyll fluorescence—an intrinsic probe of photosynthesis. In Govindjee (ed), Bioenergetics of Photosynthesis, Acad Press, New York, pp. 320–371
Satoh K and Fork DC (1983) State I-state II transitions in the green alga Scenedesmus obliquus. Photochem Photobiol 37: 429–434
Spalding MH, Spreitzer RJ and Ogren WL (1983) Carbonic anhydrase-deficient mutant of Chlamydomonas reinhardii requires elevated carbon dioxide concentrations for photoautotrophic growth. Plant Physiol 73: 268–272
Vermaas WJF and Govindjee (1981) Unique role(s) of carbon dioxide and bicarbonate in the photosynthetic electron transport system. Proc Indian Natl Sci Acad B 47: 581–605
Wild A (1979) Physioligie der Photosynthese Höherer Pflanzen. Die Anpassung an Lichtbedingungen. Ber Deutsch Bot Ges 92: 341–364
Williams WP, Furtado D and Nutbeam AR (1980) Comparison between state I/state II adaptation in a unicellular green alga and high energy state quenching in isolated intact spinach chloroplasts. Photobiochem Photobiophys 1: 91–102
Wintermans JFGM and De Mots A (1965) Spectrophotometric characteristics of chlorophyll and their pheophytins in ethanol. Biochem Biophys Acta 109: 448–453
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Spalding, M.H., Critchley, C., Govindjee et al. Influence of carbon dioxide concentration during growth on fluorescence induction characteristics of the Green Alga Chlamydomonas reinhardii . Photosynth Res 5, 169–176 (1984). https://doi.org/10.1007/BF00028529
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00028529