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Peroxidase assay in plants: Interference by ascorbic acid and endogenous inhibitors in Sedum and Pelargonium enzyme extracts

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Abstract

Sedum album and Pelargonium zonale extracts do not show any peroxidase activity. Both extracts provoke a lag phase in the horse-radish peroxidase-catalyzed oxidation of guaiacol by H2O2. Preincubation of Sedum album extract with ascorbate oxidase eliminated completely the lag phase. Ascorbic acid has been identified as the substance responsible for this lag phase by reacting with a coloured intermediary product of the analytical reaction. In the Pelargonium zonale extract, the lag phase seems to be due to competitive inhibitors of peroxidase, which are of a phenolic nature.

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Authors and Affiliations

  1. Département de Biologie Végétale, Université de Genéve, 3, Place de l'Université, CH-1211, Genéve 4, Suisse

    Federico J. Castillo, Fredi Celardin & Hubert Greppin

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  1. Federico J. Castillo
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  2. Fredi Celardin
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  3. Hubert Greppin
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Castillo, F.J., Celardin, F. & Greppin, H. Peroxidase assay in plants: Interference by ascorbic acid and endogenous inhibitors in Sedum and Pelargonium enzyme extracts. Plant Growth Regul 2, 69–75 (1984). https://doi.org/10.1007/BF00024855

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  • DOI: https://doi.org/10.1007/BF00024855

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