Skip to main content
SpringerLink
Log in

Effects of Ri plasmid rol gene expression on the IAA content of transformed roots of Solanum dulcamara L.

  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The effects have been investigated of various combinations of rol genes, together with Ri plasmid TR-DNA transcripts 1 and 2, upon free IAA levels in transformed roots of Solanum dulcamara L. Differences between the IAA levels were observed, including a highly significant (p=0.01) difference between the IAA levels of rol − TR+ and rol ABC+ TR+ transformed roots. Possible mechanisms are discussed for the regulation of free IAA levels by rol gene products.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

GUS:

β-glucuronidase

IAA:

indole-3-acetic acid

kb:

kilo-base pairs

NAA:

1-naphthaleneacetic acid

TFA/AA:

trifluoracetic acid/acetic anhydride

f.wt.:

fresh weight

References

  1. Blakesley D, Hall JF, Weston GD and Elliott MC (1983) Simultaneous analysis of indole-3-acetic acid and detection of 4-chlorindole-3-acetic acid and 5-hydroxyindole-3-acetic acid in plant tissue by high performance liquid chromatography of their 2-methyl-indolo-pyrone derivatives. J. Chromatography 258: 155–164

    Article  Google Scholar 

  2. Blakesley D, Allsopp AJA, Hall JF, Weston GD and Elliott MC (1984) Use of reversed-phase ion-pair high performance liquid chromatography for the removal of compounds inhibitory to the formation of the 2-methyl indolo- pyrone derivative of indole-3-acetic acid. J. Chromatography 294: 480–484

    Article  Google Scholar 

  3. Cardarelli M, Spano L, DePaolis A, Mauro ML, Vitalli G and Costantino P (1985) Identification of the genetic locus responsible for non-polar root induction by Agrobacterium rhizogenes. Plant Molecular Biology 5: 385–391

    Google Scholar 

  4. Davey MR, Gartland KMA and Mulligan BJ (1986) Transformation of the genomic expression of plant cells. In: DH Jennings and AJ Trewavas, eds. Plasticity in Plants 85–120. SEB Symposium XXXX, Company of Biologists, Cambridge

  5. Guern J (1989) Use of mutants and transformed plants to study the action of plant hormones. In: J Roberts and MA Venis, eds. Hormone Perception and Signal Transduction in Animals and Plants: SEB/Nottingham University Press (in press)

  6. Huffman GA, White F, Gordon MP and Nester EW (1984) Hairy-root inducing plasmid: Physical map and homology to tumour-inducing plasmids. J. Bacteriol. 157: 269–276

    PubMed  Google Scholar 

  7. Jouanin L (1984) Restriction map of an agropine type Ri plasmid and its homologies with Ti plasmids. Plasmid 12: 91–102

    PubMed  Google Scholar 

  8. Kikuchi M, Imaseki H and Sakai S (1989) Modulation of gene expression in isolated nuclei by auxin binding proteins. Plant Cell Physiol. 30: 765–773

    Google Scholar 

  9. Maurel C, Brevet J, Barbier-Brygoo H, Guern J and Tempe J (1990) Auxin regulates the promoter of the root-inducing rolB gene of Agrobacterium rhizogenes in transgenic tobacco. Mol. Gen. Genet. 223: 58–64

    Article  PubMed  Google Scholar 

  10. McInnes E (1990) Genetic analysis of the T-DNA genes of the Agrobacterium rhizogenes Ri plasmid. Ph.D. Thesis, University of Nottingham

  11. McInnes E, Davey MR, Mulligan BJ, Davies K, Sargent AW and Morgan AJ (1989) Use of a disarmed Ri plasmid vector in analysis of transformed root induction. J. Exp. Botany 40: 1135–1144

    Google Scholar 

  12. Moore L, Warren G and Strobel G (1979) Involvement of a plasmid in the hairy root disease of plants caused by Agrobacterium rhizogenes. Plasmid 2: 657–662

    Google Scholar 

  13. Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  14. Offringa LA, Melchers LS, Regensburg-Tuink AJG, Costantino P, Schilperoort RA and Hooykaas PJJ (1986) Complementation of Agrobacterium tumefaciens tumour-inducing aux mutants by genes for the TR-region of the Ri plasmid of Agrobacterium rhizogenes. Proc. Natl. Acad. Science USA 83: 6935–6959

    Google Scholar 

  15. Schmulling T, Schell J and Spena A (1989) Promoters of the rolABC genes of Agrobacterium rhizogenes are differentially regulated in transgenic plants. The Plant Cell 1: 665–670

    Article  PubMed  Google Scholar 

  16. Schroder G, Waffenschmidt S, Weiler EW and Schroder J (1984) The T-region of Ti plasmids codes for an enzyme synthesizing indole-3-acetic acid. Eur. J. Biochem. 138: 387–391

    PubMed  Google Scholar 

  17. Shen WH, Petit A, Guern J and Tempe J (1988) Hairy roots are more sensitive to auxin than normal roots. Proc. Natl. Acad. Science USA 85: 3417–3421

    Google Scholar 

  18. Slightom J (1986) Nucleotide sequence of TL-DNA of Agrobacterium rhizogenes agropine type plasmid. J. Biol. Chem. 261: 108–121

    PubMed  Google Scholar 

  19. Sokal RR and Rohlf FJ (1981) Biometry, 2nd Edition. WH Freeman and Co., Oxford and New York

    Google Scholar 

  20. Sugaya S, Hayakawa K, Handa T and Uchimiya H (1989) Cell specific expression of the rolC gene of the TL-DNA of Ri plasmid in transgenic tobacco plants. Plant Cell Physiol. 30: 649–653

    Google Scholar 

  21. Thomashow LS, Reeves S and Thomashow MF (1984) Crown gall oncogenesis: evidence that a T-DNA gene from the Agrobacterium Ti plasmid pTiA6 encodes an enzyme that catalyses synthesis of indoleacetic acid. Proc. Natl. Acad. Science USA 81: 5071–5075

    Google Scholar 

  22. White FF, Taylor BH, Huffman GA, Gordon MP and Nester EW (1985) Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes. J. Bacteriol. 164: 33–44

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. E. McInnes

    Present address: ICI Seeds, Jealott's Hill Research Station, RG12 6EY, Bracknell, Berkshire, UK

Authors and Affiliations

  1. Plant Molecular Biology Group Department of Applied Biology and Biotechnology, The David Attenborough Laboratories, Leicester Polytechnic, Scraptoft, LE7 9SU, Leicester, UK

    K. M. A. Gartland, J. F. Hall & M. C. Elliott

  2. Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, Nottingham, UK

    E. McInnes, B. J. Mulligan & M. R. Davey

  3. Hendrix-International, Postbus 220, 5830 AE, Boxmeer, The netherlands

    A. J. Morgan

Authors
  1. K. M. A. Gartland
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. McInnes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. F. Hall
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. J. Mulligan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. J. Morgan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. C. Elliott
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. R. Davey
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gartland, K.M.A., McInnes, E., Hall, J.F. et al. Effects of Ri plasmid rol gene expression on the IAA content of transformed roots of Solanum dulcamara L.. Plant Growth Regul 10, 235–241 (1991). https://doi.org/10.1007/BF00024414

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00024414

Key words

Search

Navigation