Summary
Minisatellite DNA from 13 Rubus cultivars, including five raspberries, two R. flagellaris derivatives, three R. ursinus derivatives, and the three raspberry-blackberry hybrids Boysen, Logan Thornless and Young, was hybridized with the M13 probe yielding cultivar-specific DNA fragment profiles. Estimates of similarity, assessed from the fragment profiles, showed a strong association with the degree of genetic relatedness among the cultivars. Boysen was shown to be closely related to Austin Thornless, Logan and Young, and is thus thought to be of similar origin to Young, that is Austin Thornless x Phenomenal. While genetic relatedness of Creston with Lloyd George was suggested, Lloyd George parentage of Creston cannot be confirmed from the data obtained.
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References
Bailey, L.H., 1941. Species batorum. The genus Rubus in North America I. Gentes Herb. 5: 1–64.
Britton, D.M. & J.W. Hull, 1956. Mitotic instability in black-berry seedlings. J. Hered. 47: 205–210.
Brown, S.W., 1943. The origin and nature of variability in the Pacific coast blackberries (R. ursinus Cham. & Schlecht. and R. lemurum sp. nov.). Amer. J. Bot. 30: 686–697.
Cousineau, J.C. & D.J. Donnelly, 1989. Identification of raspberry cultivars in vivo and in vitro using isoenzyme analysis. HortScience 24: 490–492.
Dallas, J.F., 1988. Detection of DNA ‘fingerprints’ of cultivated rice by hybridization with a human minisatellite probe. Proc. Natl. Acad. Sci. U.S.A. 85: 6831–6835.
Darrow, G.M., 1925. The Young dewberry, a new hybrid variety. Amer. Fruit Grower 45: 9, 33.
Darrow, G.M., 1955. Blackberry-raspberry hybrids. J. Hered. 46: 67–71.
Fejer, S.O., 1977. Inheritance of yield, yield components, and fall-fruiting habit in red raspberry diallel crosses. Can. J. Genet. Cytol. 19: 1–13.
Georges, M., A.-S. Lequarré, M. Castelli, R. Hanset & G. Vassart, 1988. DNA fingerprinting in domestic animals using four different minisatellite probes. Cytogenet. Cell Genet. 47: 127–131.
Gilbert, D.A., N. Lehman, S.J. O'Brien & R.K. Wayne, 1990. Genetic fingerprinting reflects population differentiation in the California Channel Island fox. Nature 344: 764–767.
Hall, H.K., 1990. Blackberry breeding. Plant Breeding Reviews 8.
Hillel, J., T. Schaap, A. Haberfeld, A.J. Jeffreys, Y. Plotzky, A. Cahaner & U. Lavi, 1990. DNA fingerprints applied to gene introgression in breeding programs. Genetics 124: 783–789.
Jeffreys, A.J., V. Wilson & S.L. Thein, 1985a. Hypervariable ‘minisatellite’ regions in human DNA. Nature 314: 67–73.
Jeffreys, A.J., V. Wilson & S.L. Thein, 1985b. Individual-specific ‘fingerprints’ of human DNA. Nature 316: 76–79.
Jennings, D.L., 1981. A hundred years of Loganberries. Fruit Var. J. 35: 34–37.
Jennings, D.L., 1988. Raspberries and blackberries: their breeding, diseases and growth. Academic Press, London.
Kuhnlein, U., D. Zadworny, Y. Dawe, R.W. Fairfull & J.S. Gavora, 1990. Assessment of inbreeding by DNA fingerprinting: development of a calibration curve using defined strains of chickens. Genetics 125: 161–165.
Lynch, M., 1988. Estimation of relatedness by DNA finger-printing. Mol. Biol. Evol. 5: 584–599.
Nybom, H., 1990a. DNA fingerprints in sports of ‘Red Delicious’ apples. HortScience: 1641–1642.
Nybom, H., 1990b. Genetic variation in ornamental apple trees and their seedlings (Malus, Rosaceae) revealed by DNA ‘fingerprinting’. Hereditas 113: 17–28.
Nybom, H. & S.H. Rogstad, 1990. DNA ‘fingerprinting’ detect genetic variation in Acer negundo. Plant Syst. Evol. 173: 49–56.
Nybom, H., S.H. Rogstad & B.A. Schaal, 1990. Genetic variation detected by use of the M13 ‘DNA fingerprint’ probe in Malus, Prunus, and Rubus (Rosaceae). Theor. Appl. Genet. 79: 153–156.
Nybom, H. & B.A. Schaal, 1990a. DNA ‘fingerprints’ applied to paternity analysis in apples (Malus x domestica). Theor. Appl. Genet. 79: 763–768.
Nybom, H. & B.A. Schaal, 1990b. DNA ‘fingerprints’ reveal genotypic distributions in natural populations of blackberries and raspberries. Amer. J. Bot. 77: 883–888.
Nybom, H., B.A. Schaal & S.H. Rogstad, 1989. DNA ‘fingerprints’ can distinguish cultivars of blackberries and raspherries. Acta Hortic. 262: 305–310.
Reeve, H.K., D.F. Westneat, W.A. Noon, P.W. Sherman & C.F. Aquadro, 1990. DNA ‘fingerprinting’ reveals high levels of inbreeding in colonies of the eusocial naked mole rat. Proc. Natl. Acad. Sci. U.S.A. 87: 2496–2500.
Rogstad, S.H., J.C. PattonII & B.A. Schaal, 1988. M13 repeat probe detects DNA minisatellite-like sequences in gymnosperms and angiosperms. Proc. Natl. Acad. Sci. U.S.A. 85: 9176–9178.
Ryskov, A.P., A.G. Jincharadze, M.I. Prosnyak, P.L. Ivanov & S.A. Limborska, 1988. M13 phage DNA as a universal marker for DNA fingerprinting of animals, plants and microorganisms. FEBS Letters 233: 388–392.
Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen & R.W. Allard, 1984. Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. U.S.A. 81: 8014–8018.
Sokal, R.S. & F.J. Rohlf, 1969. Biometry. W.H. Freeman & Co., San Fransisco.
Thompson, M.M., 1961. Cytogenetics of Rubus. II. Cytological studies of the varieties ‘Young’, ‘Boysen’ and related forms. Amer. J. Bot. 48: 667–673.
Waldo, G.F., 1968. Blackberry breeding involving native Pacific coast parentage. Fruit Var. Hort. Digest 22: 3–7.
Westneat, D.F., W.A. Noon, H.K. Reeve & C.F. Aquadro, 1988. Improved hybridization conditions for DNA ‘finger-prints’ probed with M13. Nucleic Acids. Res. 16: 4161.
Wetton, J.H., R.E. Carter, D.T. Parkin & D. Walters, 1987. Demographic study of a wild house sparrow population by DNA fingerprinting. Nature 327: 147–149.
Zimmerman, P.A., N. Lang-Unnasch & C.A. Cullis, 1989. Polymorphic regions in plant genomes detected by an M13 probe. Genome 32: 824–828.
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Nybom, H., Hall, H.K. Minisatellite DNA ‘fingerprints’ can distinguish Rubus cultivars and estimate their degree of relatedness. Euphytica 53, 107–114 (1991). https://doi.org/10.1007/BF00023790
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DOI: https://doi.org/10.1007/BF00023790