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Transposition pattern of a modified Ds element in tomato

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Abstract

Several aspects of transposition of an in vitro modified Ds element are described. This Ds element, designated ds-r, is equipped with bacterial plasmid sequences and can, therefore, be rescued from the plant genome. Our results indicate that the Ds-r element has a ‘late’ timing of transposition from T-DNAs. This feature of the element might be advantageous for tagging experiments because it leads to independently transposed germinally transmitted elements. Furthermore, it is shown that Ds-r transposition generates clusters of insertions, indicating that ‘genes to be tagged’ should be located in genomic regions covered by insertions.

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Author notes

  1. Caius M. T. Rommens

    Present address: Department of Plant Pathology, University of California, Berkeley, CA 94720, USA

Authors and Affiliations

  1. Department of Genetics, Free University, De Boelelaan 1087, 1081 HV, Amsterdam, Netherlands

    Caius M. T. Rommens, Tichafa R. I. Munyikwa, Bert Overduin, H. John J. Nijkamp & Jacques Hille

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  1. Caius M. T. Rommens
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  2. Tichafa R. I. Munyikwa
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  4. H. John J. Nijkamp
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  5. Jacques Hille
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Rommens, C.M.T., Munyikwa, T.R.I., Overduin, B. et al. Transposition pattern of a modified Ds element in tomato. Plant Mol Biol 21, 1109–1119 (1993). https://doi.org/10.1007/BF00023607

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  • DOI: https://doi.org/10.1007/BF00023607

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