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A pathogen-induced gene of barley encodes a HSP90 homologue showing striking similarity to vertebrate forms resident in the endoplasmic reticulum

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Abstract

The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identified as a member of the 90 kDa heat shock protein (HSP90) family. The encoded protein most resembles the endoplasmic reticulum (ER) resident HSP90 protein, the 94 kDa glucose-regulated protein (GRP94) of vertebrates, as it possesses both the characteristic N-terminal domain including a signal peptide sequence and the C-terminal ER retention signal (Lys-Asp-Glu-Leu). A transcript cross-hybridizing at high stringency accumulated rapidly in leaves upon heat shock treatment. Genomic DNA blot analysis indicated the presence of a family of related genes in the barley genome.

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Authors and Affiliations

  1. Section for Plant Pathology, Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsenvej 40, DK-1871, Frederiksberg C, Copenhagen, Denmark

    Haidee Walther-Larsen, Jakob Brandt, David B. Collinge & Hans Thordal-Christensen

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  1. Haidee Walther-Larsen
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  2. Jakob Brandt
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  3. David B. Collinge
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  4. Hans Thordal-Christensen
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Walther-Larsen, H., Brandt, J., Collinge, D.B. et al. A pathogen-induced gene of barley encodes a HSP90 homologue showing striking similarity to vertebrate forms resident in the endoplasmic reticulum. Plant Mol Biol 21, 1097–1108 (1993). https://doi.org/10.1007/BF00023606

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  • DOI: https://doi.org/10.1007/BF00023606

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