Abstract
A collection of Cannabis accessions was evaluated for resistance to the root-knot nematode Meloidogyne hapla Chitwood in a seedling test. After inoculation with a larval suspension, significant variation was found for the number of galls and egg masses on the seedling roots. These parameters were considered as estimates for nematode infection and larval multiplication, respectively. A subset of the tested accessions was grown on a naturally infested arable field to study the relation between the test results and host characteristics in the field. The ranking order of accessions for the number of galls in the seedling test agreed well with that for the number of root galls on juvenile plants in the field. Therefore the test provides a useful indication for nematode infection. The number of egg masses in the seedling test and the population density of Meloidogyne measured in post-treatment soil samples of the field trial, showed a comparable ranking of accessions. Differences between accessions in the field were however smaller and not statistically significant.
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References
Barker, K.R., C.C. Carter & J.N. Sasser, 1985. An Advanced Treatise on Meloidogyne, Volume II: Methodology. Cooperative Publication of the Department of Plant Pathology and the US Agency for International Development. North Carolina State University Graphics.
Boukema I.W., K. Hofman, H. Toxopeus & F.W.M. Lamers, 1983. An efficient screening method for root knot nematode resistance in cucurbits and crucifers. In: W. Lange, A.C. Zeven & N.G. Hogenboom (eds.). Efficiency in plant breeding: proceedings of the 10th Congress of the European Association for Research on Plant Breeding, pp. 299. Pudoc, Wageningen.
Boukema I.W., G.T.M. Reuling & K. Hofman, 1984. The reliability of a seedling test for resistance to root-knot nematodes in cucurbits. Cucurbit Genetics Coop. Rpt. 7: 92–93.
Hall, H.C. van, 1828. Verhandeling, inhoudende ene beschrijving van de hennep-teelt in Nederland. [Description of hemp cultivation in the Netherlands.] [Dutch]. J. Oomkens, Groningen.
s'Jacob, J.J. & J. van Bezooijen, 1984. A manual for practical work in nematology. Revised (1984) edition. Department of Nematology, Agricultural University of Wageningen.
Lubberts J.H. & H. Toxopeus. 1982. Het ontwikkelen van bietecystenaaltjes-resistente bladramenas en gele mosterd. [The development of beet cyst nematode resistant fodder radish and white mustard.] [Dutch]. Zaadbelangen 36: 66–69.
Meijer E.P.M. de & L.J.M. van Soest, 1992. The CPRO Cannabis germplasm collection. Euphytica 62: 201–211.
Nijs A.P.M. den & K. Hofman. 1983. An efficient procedure to screen for resistance to root-knot nematodes in cucurbits. Cucurbit Genetics Coop. Rpt. 6: 96–98.
Norton D.C., 1966. Additions to the known hosts of Meloidogyne hapla. Plant disease reporter. 50: 523–524.
Snellen J.B., 1853. De teelt van de Hennep. [The cultivation of hemp.] [Dutch]. Landbouw-courant 7: 47–48.
Zondervan A.A.W., 1987. Ontwikkeling van een biotoets voorhet Noordelijk wortelknobbelaaltje (Meloidogyne hapla). [The development of a bioassay for Meloidogyne hapla.] [Dutch]. Verslag nr. 70, PAGV, Lelystad.
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de Meijer, E.P.M. Evaluation and verification of resistance to Meloidogyne hapla Chitwood in a Cannabis germplasm collection. Euphytica 71, 49–56 (1993). https://doi.org/10.1007/BF00023466
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DOI: https://doi.org/10.1007/BF00023466