Abstract
A defective S-allele, S 0, and a functional S-allele, S x, have previously been found to be retained in an F1 hybrid of a self-compatible commercial cultivar of Petunia hybrida. Pistil proteins associated with these two alleles have also been identified. Their amino-terminal sequences have been found to share a high degree of similarity with those of S-proteins characterized from self-incompatible solanaceous species. Here we report the isolation and sequencing of cDNAs encoding S 0- and S x-proteins. Their deduced amino acid sequences contain all the consensus primary structural features of S-proteins from self-incompatible solanaceous species. Both proteins also have ribonuclease activity. The implications of these findings are discussed in relation to the presumed function of the S-protein in the self-incompatibility interaction.
This is a preview of subscription content, log in via an institution to check access.
References
Ai Y, Singh A, Coleman CE, Ioerger TR, Kheyr-Pour A, Kao T-h. Self-incompatibility in Petunia inflata: isolation and characterization of cDNAs encoding three S-allele-associated proteins. Sex Plant Reprod 3: 130–138 (1990).
Ai Y, Kron E, Kao T-h: S-alleles are retained and expressed in self-compatible cultivar of Petunia hybrida. Mol Gen Genet 230: 353–358 (1991).
Anderson MA, McFadden GI, Bernatzky R, Atkinson A, Orpin T, Dedman H, Tregear G, Fernley R, Clarke AE: Sequence variability of three alleles of the self-incompatibility gene of Nicotiana alata. Plant Cell 1: 483–491 (1989).
Blank A, Sugiyama RH, Dekker CA: Activity staining of nucleolytic enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of aqueous isopropanol to remove detergent from gels. Anal Biochem 120: 267–275 (1982).
Broothaerts W, Vanvinckenroye P, Decock B, Van Damme J, Vendrig JC: Petunia hybrida S-proteins: ribonuclease activity and the role of their glycan side chains in self-incompatibility. Sex Plant Reprod 4: 258–266 (1991).
Clark KR, Okule JJ, Collins PD, Sims TL: Sequence variability and developmental expression of S-alleles in self-incompatible and pseudoself-compatible Petunia. Plant Cell 2: 815–826 (1990).
Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, Dooner H, Bedrook J: mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo. Nucl Acids Res 14: 2229–2240 (1986).
Frankel R, Galun E: Pollination Mechanisms, Reproduction and Plant Breeding. Springer-Verlag Berlin/Heidelberg/New York (1977).
Gray JE, McClure BA, Bonig I, Anderson MA, Clarke AE: Action of the style product of the self-incompatibility gene of Nicotiana alata (S-RNase) on in vitro-grown pollen tubes. Plant Cell 3: 271–283 (1991).
Ioerger TR, Gohlke JR, Xu B, Kao T-h: Primary structural features of the self-incompatibility protein in Solanaceae. Sex Plant Reprod 4: 81–87 (1991).
Kaufmann H, Salamini F, Thompson RD: Sequence variability and gene structure at the self-incompatibility locus of Solanum tuberosum. Mol Gen Genet 226: 457–466 (1991).
Kheyr-Pour A, Bintrim SB, Ioerger TR, Remy R, Hammond SA, Kao T-H: Sequence diversity of pistil S-proteins associated with gametophytic self-incompatibility in Nicotiana alata. Sex Plant Reprod 3: 88–97 (1990).
Lewis D: Genetic control of specificity and activity of the S antigen in plants. Proc Roy Soc Lond Ser B 151: 468–477 (1960).
McClure BA, Haring V, Ebert PR, Anderson MA, Simpson RJ, Sakiyama F, Clarke AE: Style self-incompatibility gene products of Nicotiana alata are ribonucleases. Nature 342: 955–957 (1989).
McClure BA, Gray JE, Anderson MA, Clarke AE: Self-incompatibility in Nicotiana alata involves degradation of pollen rRNA. Nature 347: 757–760 (1990).
de Nettancourt D: Incompatibility in Angiosperms. Springer-Verlag, Berlin/Heidelberg/New York (1977).
Singh A, Ai Y, Kao T-h: Characterization of ribonuclease activity of three S-allele-associated proteins of Petunia inflata. Plant Physiol 96: 61–68 (1991).
Sink KC: Taxonomy. In: Sink KC (ed) Petunia, pp. 3–9. Springer-Verlag, Berlin/Heidelberg/New York (1984).
Whitehouse HLK: Multiple allelomorph incompatibility of pollen and style in the evolution of the angiosperms. Ann Bot 14: 199–216 (1950).
Xu B, Mu J, Nevins DL, Grun P, Kao T-h: Cloning and sequencing of cDNAs encoding two self-incompatibility associated proteins in Solanum chacoense. Mol Gen Genet 224: 341–346 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ai, Y., Tsai, DS. & Kao, Th. Cloning and sequencing of cDNAs encoding two SSS. Plant Mol Biol 19, 523–528 (1992). https://doi.org/10.1007/BF00023404
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00023404