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In vitro selection for improved chlorsulfuron tolerance in barley (Hordeum vulgare L.)

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Summary

A study was conducted to determine if the chlorsulfuron tolerance existing in barley could be improved with the use of in vitro tissue culture. Differential herbicide concentration led to variable culture response for callus culture.

Plants regenerated from callus culture were evaluated for chlorsulfuron tolerance in the greenhouse. Regenerants showed improved tolerance when compared to the control or unselected tissue culture-derived lines.

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Authors and Affiliations

  1. Plant Biotechnology Institute, 110 Gymnasium Place, S7N 0W9, Saskatoon, SK, Canada

    A. M. R. Baillie & K. K. Kartha

  2. Crop Development Centre, University of Saskatchewan, S7N 0W0, Saskatoon, SK, Canada

    B. G. Rossnagel

Authors
  1. A. M. R. Baillie
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  2. B. G. Rossnagel
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  3. K. K. Kartha
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Baillie, A.M.R., Rossnagel, B.G. & Kartha, K.K. In vitro selection for improved chlorsulfuron tolerance in barley (Hordeum vulgare L.). Euphytica 67, 151–154 (1993). https://doi.org/10.1007/BF00022738

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  • DOI: https://doi.org/10.1007/BF00022738

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