Skip to main content
SpringerLink
Log in

Expression analysis of a sucrose synthase gene from sugar beet (Beta vulgaris L.)

  • Research Article
  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

To investigate the expression pattern of sucrose synthase, a cDNA from tap roots of sugar beet (Beta vulgaris L.) was isolated using a heterologous sucrose synthase cDNA from potato. The 2762 bp long cDNA clone designated SBSS 1 encodes for a 822 amino acid polypeptide of a predicted molecular mass of 93.7 kDa. The deduced amino acid sequence of sugar beet sucrose synthase has homologies of 65–70% when compared to predicted amino acid sequences of sucrose synthases from other species. RNA blot analysis shows that SBSS1 is expressed most predominant in tap root under normal growth conditions. Cold treatment and anaerobiosis lead to an increase in the steady-state levels of SBSS 1 mRNA in leaf and root tissue. In tap root slices, sugars in various concentrations had no influence on the SBSS 1 transcript level. On the other hand, wounding resulted in a decreased transcript level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Arai M, Mori H, Imaseki H: Expression of the gene for sucrose synthase during growth of mung bean seedlings. Plant Cell Physiol 33: 503–506 (1992).

    Google Scholar 

  2. Avigad G: Sucrose and other disaccharides. In: Loewus FA, Tanner W (eds) Encyclopedia of Plant Physiology New Series, 13.A, pp. 217–347. Springer-Verlag Berlin (1982).

    Google Scholar 

  3. Chen YC, Chourey PS: Spatial and temporal expression of the two sucrose synthase genes in maize: immunohistological evidence. Theor Appl Genet 78: 553–559 (1989).

    Article  Google Scholar 

  4. Chopra S, Delfavero J, Dolferus R, Jacobs M: Sucrose synthase of Arabidopsis: genomic cloning and sequence characterization. Plant Mol Biol 18: 131–134 (1992).

    Article  PubMed  Google Scholar 

  5. Chourey PS, Nelson OE: The enzymatic deficiency conditioned by the shrunken-1 mutation in maize. Biochem Genet 14: 1041–1055 (1976).

    PubMed  Google Scholar 

  6. Chourey PS: Genetic control of sucrose synthetase in maize endosperm. Mol Gen Genet 184: 372–376 (1981).

    Google Scholar 

  7. Chourey PS, Latham MD, Still PE: Expression of two sucrose synthase genes in endosperm and seedling cells of maize: evidence of tissue specific polymerization of promoters. Mol Gen Genet 203: 251–255 (1986).

    Article  Google Scholar 

  8. Chourey PS, DeRobertis GA, Still PE: Altered tissue specificity of the revertant shrunken allele upon Dissociation (DS) excision is associated with loss of expression and molecular rearrangement at the corresponding nonallelic isozyme locus in maize. Mol Gen Genet 214: 300–306 (1988).

    Article  Google Scholar 

  9. Chourey PS, Chen Y-C, Miller ME: Early cell degeneration in developing endosperm is unique to the shrunken mutation in maize. Maydica 36: 141–146 (1991).

    Google Scholar 

  10. Crespi MD, Zabaleta EJ, Pontis HG, Salerno GL: Sucrose synthase expression during cold acclimation in wheat. Plant Physiol 96: 887–891 (1991).

    Google Scholar 

  11. Fieuw S, Willenbrink J: Sugar transport and sugarmetabolizing enzymes in sugar beet storage roots (Beta vulgaris ssp. altissima). J Plant Physiol 137: 216–223 (1990).

    Google Scholar 

  12. Fu H, Du J, Park WD: Cloning and sequencing of two differentially expressed sucrose synthase genes from potato. Third International Congress on Plant Mol biology, Tucson, Abstract 705 (1991).

  13. Gubler U, Hoffman BJ: A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269 (1983).

    Article  PubMed  Google Scholar 

  14. Heim U, Weber H, Bäumlein H, Wobus U: A sucrosesynthase gene of Vicia faba L.: expression pattern in developing seeds in relation no starch synthesis and metabolic regulation. Planta 191: 394–401 (1993).

    PubMed  Google Scholar 

  15. Heinlein M, Starlinger P: Tissue-and cell-specific expression of the two sucrose synthase isoenzymes in developping maize kernels. Mol Gen Genet 215: 441–446 (1989).

    Google Scholar 

  16. Hesse H, Sonnewald U, Willmitzer L: Cloning and expression analysis of sucrose-phosphate synthase from sugar beet (Beta vulgaris L.). Mol Gen Genet 247: 515–520 (1995).

    Article  PubMed  Google Scholar 

  17. Huang X-F, Nguyen-Quoc B, Chourey PS, Yelle S: Complete nucleotide sequence of the maize (Zea mays L.) sucrose synthase 2 cDNA. Plant Physiol 104: 293–294 (1994).

    Article  PubMed  Google Scholar 

  18. Koch KE, Nolte KD, Duke ER, McCarty DR, Avigne WT: Sugar levels modulate differential expression of maize sucrose synthase genes. Plant Cell 4: 59–69 (1992).

    Article  PubMed  Google Scholar 

  19. Kumar AS, Moore PH, Maretzki A: Amplification and cloning of sugarcane sucrose synthase cDNA by anchored PCR. PCR Meth Appl 2: 70–75 (1992).

    Google Scholar 

  20. Logemann J, Schell J, Willmitzer L: Improved method for the isolation of RNA from plant tissue. Anal Biochem 163: 16–20 (1987).

    PubMed  Google Scholar 

  21. Logemann J, Mayer JE, Schell J, Willmitzer L: Differential expression of genes in potato tubers after wounding. Proc Natl Acad Sci USA 85: 1136–1140 (1988).

    Google Scholar 

  22. Maas C, Schaal S, Werr W: A feedback control element near the transcription start site of the maize Shrunken gene determines promoter activity. EMBO J 9: 3447–3452 (1990).

    PubMed  Google Scholar 

  23. Martin T, Frommer WB, Salanoubat M, Willmitzer L: Expression of an Arabidopsis sucrose synthase gene indicates a role in metabolization of sucrose both during phloem loading and sink organs. Plant J 4: 367–377 (1993).

    Article  PubMed  Google Scholar 

  24. Maraña C, Garcia-Olmedo F, Carbonero P: Different expression of two types of sucrose synthase-encoding genes in wheat in response to anaerobiosis, cold shock and light. Gene 88: 167–172 (1990).

    Article  PubMed  Google Scholar 

  25. McCarty DR, Shaw JR, Hannah LC: The cloning, genetic mapping, and expression of the constitutive sucrose synthase locus of maize. Proc Natl Acad Sci USA 83: 9099–9103 (1986).

    Google Scholar 

  26. McElfresh KC, Chourey PS: Anaerobiosis induces transcription but not translation of sucrose synthase in maize. Plant Physiol 87: 542–546 (1988).

    Google Scholar 

  27. Müller-Röber BT, Koßmann J, Hannah LC, Willmitzer L, Sonnewald U: One of two different ADP-glucose pyrophosphorylase genes from potato responds strongly to elevated levels of sucrose. Mol Gen Genet 224: 136–146 (1990).

    Article  PubMed  Google Scholar 

  28. Nakamura K, Ohto M, Yoshida N, Nakamua K: Sucrose-induced accumulation of β-amylase occurs concomitant with the accumulation of starch and sporamin in leafpetiole cuttings of sweet potato. Plant Physiol 96: 902–909 (1991).

    Google Scholar 

  29. Ricard B, Rivoal J, Spiteri A, Pradet A: Anaerobic stress induces the transcription and translation of sucrose synthase in rice. Plant Physiol 95: 669–674 (1991).

    Google Scholar 

  30. Rowland LJ, Chen YC, Chourey PS: Anaerobic treatments alters the cell specific expression of Adh-1, Sh, and Sus genes in roots of maize seedlings. Mol Gen Genet 218: 33–40 (1989).

    Article  Google Scholar 

  31. Salanoubat M, Belliard G: Molecular cloning and sequencing of sucrose synthase cDNA from potato (Solanum tuberosum L.): prelimenary characterization of sucrose synthase mRNA distribution. Gene 60: 47–56 (1987).

    Article  PubMed  Google Scholar 

  32. Salanoubat M, Belliard G: The steady-state level of potato sucrose synthase mRNA is dependent on wounding, anaerobiosis and sucrose concentration. Gene 84: 181–185 (1989).

    Article  PubMed  Google Scholar 

  33. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).

    Google Scholar 

  34. Sharrock RE, Kramer M, Koorneef M, Quail PH: Molecular analysis of phytochrome deficiency in an aurea mutant of tomato. Mol Gen Genet 213: 9–14 (1988).

    Article  Google Scholar 

  35. Sun JD, Loboda T, Sung SJS, Black CC: Sucrose synthase in wild tomato, Lycopersicon chmielewskii, and tomato fruit sink strength. Plant Physiol 98: 1117–1121 (1992).

    Google Scholar 

  36. Sung S-JS, Xu DP, Black CC: Identification of actively filling sucrose sinks. Plant Physiol 89: 1117–1121.

  37. Taliercio EW, Chourey PS: Post-transcriptional control of sucrose synthase expression in aerobic seedlings of maize. Plant Physiol 90: 1359–1364 (1989).

    Google Scholar 

  38. Visser RGF, Stolte A, Jakobson E: Expression of a chimaeric granule-bound starch synthase-GUS gene in transgenic plants. Plant Mol Biol 17: 691–699 (1991).

    PubMed  Google Scholar 

  39. Wang A-Y, Yu W-P, Juang R-H, Huang J-W, Sung H-Y, Su J-C: Presence of three rice sucrose synthase genes as revealed by cloning and sequencing of cDNA. Plant Mol Biol 18: 1191–1194 (1992).

    PubMed  Google Scholar 

  40. Wang M-B, Boulter D, Gatehouse JA: A complete sequence of the rice sucrose synthase-1 (RSs1) gene. Plant Mol Biol 19: 881–885 (1992).

    Article  PubMed  Google Scholar 

  41. Werr W, Frommer WB, Maas C, Starlinger P: Structure of the sucrose synthase gene on chromosome 9 of Zea mays L. EMBO J 4: 1373–1380 (1985).

    Google Scholar 

  42. Yu WP, Wang AY, Juang RH, Sung HY, Su JC: Isolation and sequences of rice sucrose synthase cDNA and genomic DNA. Plant Mol Biol 18: 139–142 (1992).

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, 14195, Berlin, Germany

    Holger Hesse & Lothar Willmitzer

Authors
  1. Holger Hesse
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lothar Willmitzer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hesse, H., Willmitzer, L. Expression analysis of a sucrose synthase gene from sugar beet (Beta vulgaris L.). Plant Mol Biol 30, 863–872 (1996). https://doi.org/10.1007/BF00020799

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00020799

Key words

Search

Navigation