Abstract
We isolated an almost full-length cDNA clone containing β-tubulin gene from a partial cDNA library of mung bean using chicken cDNA as probe. Cross-hybridization with chicken β-tubulin cDNA and positive hybridization-selection and translation of mung bean mRNA established that this clone contains β-tubulin sequences. We studied the organization of tubulin genes in mung bean. In this plant tubulin genes are organized in tandem repeats of alternating α- and β-tubulin genes. The 5.6 kb basis repeat unit which contains both α- and β-tubulin genes is repeated twenty times per haploid genome.
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Abbreviations
- SDS:
-
sodium dodecyl sulphate
- 1×SSPE:
-
150 mM NaCl, 10 mM NaH2PO4 and 1 mM EDTA, pH 7.4
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Raha, D., Sen, K. & Biswas, B.B. cDNA cloning of β-tubulin gene and organization of tubulin genes in Vigna radiata (mung bean) genome. Plant Mol Biol 9, 565–571 (1987). https://doi.org/10.1007/BF00020533
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DOI: https://doi.org/10.1007/BF00020533