Abstract
The FB iron-sulfur cluster is destroyed preferentially by treating Photosystem I complexes with HgCl2(Kojima Y, Niinomi Y, Tsuboi S, Hiyama T and Sakurai H (1987) Bot Mag 100: 243–53). When FB is 95% depleted but FAis quantitatively retained in cyanobacterial PS I complexes, the reduction potential of FA remains highly electronegative (Em=−530 mV, n=1), the EPR spectral and spin relaxation properties of FA and FXremain unchanged, but NADP+ photoreduction rates decline from 552 to 72 μmol mg Chl−1 h−1.When FB is reconstituted with FeCl3, Na2S and β-mercaptoethanol, NADP+photoreduction rates recover to 528 μmol mg Chl−1 h−1. The correlation between the presence of FBand NADP+ photoreduction provides direct experimental evidence that this iron-sulfur cluster is required for electron throughput from cytochromec 6 to flavodoxin or ferredoxin in Photosystem I.
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Abbreviations
- Chl:
-
chlorophyll
- DPIP:
-
dichlorophenolindophenol
- PS I:
-
Photosystem I
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Published as Journal Series #11091 of the University of Nebraska Agricultural Research Division. This paper is dedicated to the memory of the late Professor Daniel Arnon, who is remembered for his gracious and generous encouragement of the senior author's early career.
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Jung, YS., Yu, L. & Golbeck, J.H. Reconstitution of iron-sulfur center FB results in complete restoration of NADP+ photoreduction in Hg-treated Photosystem I complexes from Synechococcus sp. PCC 6301. Photosynth Res 46, 249–255 (1995). https://doi.org/10.1007/BF00020437
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DOI: https://doi.org/10.1007/BF00020437