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Synthesis of ribosomal proteins from stored mRNAs early in seed germination

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Abstract

In several eukaryotic organisms the mRNA expression for ribosomal proteins (RPs) is highly regulated at the translational level. The present work addresses the synthesis of RPs early in germination and particularly the origin of their transcripts. Excised maize axes were labeled with [35S]methionine for 6 h, and both cytoplasmic and ribosomal proteins were analyzed by electrophoresis and fluorography. It was found that some RPs are newly synthesized and already incorporated into ribosomes during this period. Synthesis of RPs was also observed under conditions of transcription inhibition, suggesting the presence of stored-RP transcripts in the embryonic axes. In vitro translation of polysomes isolated from α-amanitin-treated axes early in germination (3 and 9 h) was performed. Immunoprecipitation of the products revealed the synthesis of rps. Four heterologous probes two each of the small subunit (S4 and S6) and large (L3, L16) subunit rps were used to detect the correspondent transcripts within the stored mRNA stock of the embryonic axes. Both, by slot blot and northern analysis, a positive cross-reaction occurred for all the tested samples. Although only two of them (S4 and S6) seem to be stored as mature mRNA.

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References

  1. Abernethy RH, Thiel DS, Petersen NS, Helm K: Thermotolerance is developmentally dependent in germinating wheat seed. Plant Physiol 89: 569–576 (1989).

    Google Scholar 

  2. Belandia B, Brautigan D, Martin-Perez J: Attenuation of ribosomal protein S6 phosphatase activity in chicken embryo fibroblasts transformed by rous sarcoma virus. Mol Cell Biol 14: 200–206 (1994).

    PubMed  Google Scholar 

  3. Caffarelli E, Fragapane P, Gehring C, Bozzoni I: The accumulation of mature RNA for the Xenopus laevis ribosomal protein L1 is controlled at the level of splicing and turnover of the precursor RNA. EMBO J 6: 3493–3498 (1987).

    PubMed  Google Scholar 

  4. Chan YL, Devi KRG, Olvera J, Wool IG: The primary structure of rat ribosomal protein S3. Arch Biochem Biophys 283: 546–550 (1990).

    PubMed  Google Scholar 

  5. Fehling E, Weidner M: Adaptative potential of wheat ribosomes toward heat depends on the large ribosomal subunit and ribosomal protein phosphorylation. Plant Physiol 87: 562–565 (1988).

    Google Scholar 

  6. Hershey JW: Translational control in mammalian cells. Annu Rev Biochem 60: 717–755 (1991).

    Article  PubMed  Google Scholar 

  7. Kermode AR: Regulatory mechanisms involved in the transition from seed development to germination. CRC Crit Rev Plant Sci 9: 155–195 (1990).

    Google Scholar 

  8. Krystosek A, Cawton ML, Kabat D: Improved methods for purification and assay of eucaryotic messenger ribonucleic acids and ribosomes. J Biol Chem 250: 6077–6082 (1975).

    PubMed  Google Scholar 

  9. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 231–234 (1970).

    Google Scholar 

  10. Laine RO, Shay NC, Kilberg MS: Nuclear retention of the induced mRNA following amino acid dependent transcriptional regulation of mammalian ribosomal proteins L13, S25. J Biol Chem 269: 9693–9697 (1994).

    PubMed  Google Scholar 

  11. Levy S, Avni D, Hariharan N, Perry RP, Meyuhas O: Olygopyrimidine tract of the 5′ end of mammalian ribosomal protein mRNAs is required for their translational control. Proc Natl Acad Sci USA 88: 3319–3323 (1991).

    PubMed  Google Scholar 

  12. Loreni F, Amaldi F: Translational regulation of ribosomal protein synthesis in Xenopus cultured cells: mRNA relocation between polysomes and RNP during nutritional shifts. Eur J Biochem 205: 1027–1032 (1992).

    PubMed  Google Scholar 

  13. MacNabb S, Ashburner M: Identification of a Drosophila protein similar to rat S13 and archaebacterial S11 ribosomal proteins. Nucl Acids Res 21: 2516 (1993).

    PubMed  Google Scholar 

  14. Maeda N, Kenmochi N, Tanaka T: The complete nucleotide sequence of chicken ribosomal protein L7a gene and the multiple factor binding sites in its 5′-flanking region. Biochimie 75: 785–790 (1993).

    Article  PubMed  Google Scholar 

  15. Mager WH: Control of ribosomal protein gene expression. Biochem Biophys Acta 949: 1–15 (1988).

    PubMed  Google Scholar 

  16. Mayer A, Poljakoff-Mayber MG: The Germination of Seeds. Pergamon Press, Oxford (1989).

    Google Scholar 

  17. Murahige T, Skoog C: A revised medium for rapid growth and bioassay with tobacco cultures. Physiol Plant 15: 473–479 (1962).

    Google Scholar 

  18. Naranda T, Ballesta JPG: Phosphorylation controls binding of acidic proteins to the ribosome. Proc Natl Acad Sci USA 88: 10563–10567 (1991).

    PubMed  Google Scholar 

  19. Nierhaus KH: The assembly of prokaryotic ribosomes. Biochimie 73: 739–755 (1991).

    Article  PubMed  Google Scholar 

  20. Nowotny V, Nierhaus KH: Assembly of the 30S subunit from Escherichia coli ribosomes occurs via two assembly domains which are initiated by S4 and S7. Biochemistry 27: 7051–7055 (1988).

    PubMed  Google Scholar 

  21. Pérez-Méndez A, Aguilar R, Briones E, Sánchez de Jiménez E: Characterization of ribosomal protein phosphorylation in maize axes during germination. Plant Sci 94: 71–79 (1993).

    Article  Google Scholar 

  22. Pramanik SK, Krochko JE, Bewley JD: Distribution of cytosolic mRNAs between polysomal and ribonucleo-protein complex fractions in alfalfa embryos. Plant Physiol 99: 1590–1596 (1992).

    Google Scholar 

  23. Rushton PJ, Bray CM: Stored and de novo synthesized polyadenylated RNA and loss of vigor and viability in wheat seeds. Plant Sci 51: 51–59 (1987).

    Article  Google Scholar 

  24. Sanchez de Jimenez E, Aguilar R, Lopez S: Distinctive characteristics of protein synthesis in maize embryos during early stages of germination. Biochem Biophys Res Comm 99: 445–450 (1981).

    PubMed  Google Scholar 

  25. Sanchez de Jimenez E, Aguilar R: Protein synthesis patterns: relevance of old and new messenger RNA in germinating maize embryos. Plant Physiol 75: 231–234 (1984).

    Google Scholar 

  26. Spiegel S, Marcus A: Polyribosome formation in early wheat embryo germination is independent of either transcription or polyadenylation. Nature 256: 228–230 (1975).

    PubMed  Google Scholar 

  27. Spiegel S, Obendorf RL, Marcus A: Transcription of ribosomal and messenger RNAs in early wheat embryo germination. Plant Physiol 56: 502–507 (1975).

    Google Scholar 

  28. Standart N: The RNA-protein partners in mRNP. Mol Biol Rep 18: 135–142 (1993).

    PubMed  Google Scholar 

  29. Steel LF, Jacobson A: Translational control of ribosomal protein synthesis during early Dictyostelium discoideum development. Mol Cell Biol 7: 965–972 (1987).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Departamento de Bioquímica, Conjunto E, Facultad de Química, UNAM, D.F. 04510, México, México

    Elda Beltrán-Peña, Adriana Ortíz-López & Estela Sánchez de Jiménez

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  1. Elda Beltrán-Peña
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  2. Adriana Ortíz-López
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  3. Estela Sánchez de Jiménez
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Beltrán-Peña, E., Ortíz-López, A. & de Jiménez, E.S. Synthesis of ribosomal proteins from stored mRNAs early in seed germination. Plant Mol Biol 28, 327–336 (1995). https://doi.org/10.1007/BF00020251

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  • DOI: https://doi.org/10.1007/BF00020251

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