Abstract
A full-length cDNA clone encoding apple (Malus domesticus) polyphenol oxidase (PPO) was isolated from a fruit peel cDNA library. Southern analysis indicated that apple PPO is encoded by a divergent multigene family. By northern analysis, PPO mRNA was only detected in a fruit sample taken one week after full bloom. PPO mRNA accumulated in wounded tissues, and also in peel tissue showing the symptoms of superficial scald, a post-harvest disorder. The induction of PPO mRNA provides the first evidence for transcriptional control of PPO expression after wounding or the manifestation of a physiological disorder.
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References
Cary JW, Lax AR, Flurkey WH: Cloning and characterization of cDNAs coding for Vicia faba polyphenol oxidase. Plant Mol Biol 20: 245–253 (1992).
Castresana C, deCarvalho F, Gheysen G, Habets M, Inzé D, VanMontagu M: Tissue-specific and pathogen-induced regulation of a Nicotiana plumbaginifolia β-1,3-glucanase gene. Plant Cell 2: 1131–1143 (1990).
Chen Z, Silva H, Klessig DF: Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid. Science 262: 1883–1886 (1993).
Hennig J, Dewey RE, Cutt JR, Klessig DF: Pathogen, salicylic acid and developmental dependent expression of a β-1,3-glucanase/GUS gene fusion in transgenic tobacco plants. Plant J 4: 481–493 (1993).
Hunt MD, Eannetta NT, Yu H, Newman SM, Steffens JC: cDNA cloning and expression of potato polyphenol oxidase. Plant Mol Biol 21: 59–68 (1993).
Ingle M, D'Souza MC: Physiology and control of superficial scald and apples: a review. HortScience 24: 28–31 (1989).
Keegstra K, Olsen LJ, Theg SM: Chloroplastic precursors and their transport across the envelope membranes. Annu Rev Plant Physiol Mol Biol 40: 471–501 (1989).
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685 (1970).
Mayer AM: Polyphenol oxidases in plants — recent progress. Phytochemistry 26: 11–20 (1987).
Mayer AM, Harel E: Review: polyphenol oxidases in plants. Phytochemistry 18: 193–215 (1979).
Newman SM, Eannetta NT, Yu H, Prince JP, deVicente MC, Tanksley SD, Steffens JC: Organisation of the tomato polyphenol oxidase gene family. Plant Mol Biol 21: 1035–1051 (1993).
Rathjen AH, Robinson SP: Aberrant processing of polyphenol oxidase in a variegated grapevine mutant. Plant Physiol 99: 1619–1625 (1992).
Roby D, Broglie K, Cressman R, Biddle P, Chet I, Broglie R: Activation of a bean chitinase promoter in transgenic tobacco plants by phytopathogenic fungi. Plant Cell 2: 999–1007 (1990).
Salveit MEJr: Procedures for extracting and analysing internal gas samples from plant tissues by gas chromatography. HortScience 17: 878–881 (1982).
Samac DA, Shah DM: Developmental and pathogen-induced activation of the Arabidopsis acidic chitinase promoter. Plant Cell 3: 1063–1072 (1991).
Shahar T, Hennig N, Gutfinger T, Hareven D, Lifschitz E: The tomato 66.3-kD polyphenoloxidase gene: molecular identification and developmental expression. Plant Cell 4: 135–147 (1992).
Vaughn KC, Duke SO: Function of polyphenol oxidase in higher plants. Physiol Plant 60: 106–112 (1984).
Vaughn KC, Lax AR, Duke SO: Polyphenol oxidase: the chloroplast enzyme with no established function. Physiol Plant 72: 659–665 (1988).
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Boss, P.K., Gardner, R.C., Janssen, BJ. et al. An apple polyphenol oxidase cDNA is up-regulated in wounded tissues. Plant Mol Biol 27, 429–433 (1995). https://doi.org/10.1007/BF00020197
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DOI: https://doi.org/10.1007/BF00020197