Abstract
A geranylgeranyl pyrophosphate synthase (GGPPS) gene from Capsicum annuum (bell pepper) was cloned. The nucleotide sequence shows that this gene, like the capsanthin/capsorubin gene but unlike the phytoene synthase gene from C. annuum, is not interrupted by an intron. Southern blot analysis of C. annuum genomic DNA suggests the presence of a single gene highly similar to the cDNA and also of additional related sequences. The present data suggest that this cloned gene is functional.
This is a preview of subscription content, log in via an institution to check access.
References
Aracri B, Bartley GE, Scolnik PA, Giuliano G: Sequence of the phytoene desaturase locus of tomato. Plant Physiol 106, in press (1994).
Bartley GE, Scolnik PA, Giuliano G: Molecular biology of carotenoid biosynthesis in plants. Annu Rev Plant Physiol Plant Mol Biol 45: 287–301 (1994).
Church GM, Gilbert W: Genomic sequencing. Proc Natl Acad Sci USA 81: 1991–1995 (1984).
Bouvier F, Hugueney P, d'Harlingue A, Kuntz M, Camara B: Xanthophyll biosynthesis in chromoplasts: isolation and molecular cloning of an enzyme catalyzing the conversion of 5,6-epoxycarotenoid into ketocarotenoid. Plant J 6: 45–54 (1994).
Deruère J, Bouvier F, Steppuhn J, Klein A., Camara B, Kuntz M: Structure and expression of two plant genes encoding chromoplastspecific proteins: occurence of partially spliced transcripts. Biochem Biophys Res Commun 199: 1144–1150 (1994).
Deruère J, Römer S, d'Harlingue A, Backhaus RA, Kuntz M, Camara B: Fibril assembly and carotenoid overaccumulation in chromoplasts: a model for supramolecular lipoprotein structures. Plant Cell 6: 119–133 (1994).
Dogbo O, Camara B: Purification of isopentenyl isomerase and geranylgeranyl pyrophosphate synthase from Capsicum chromoplasts by affinity chromatography. Biochim Biophys Acta 920: 140–148 (1987).
Hugueney P, Römer S, Kuntz M, Camara B: Characterization and molecular cloning of a flavoprotein catalyzing the synthesis of phytofluence and ζ-carotene in Capsicum chromoplasts (1992).
Jefferson RA: Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5: 387–405 (1987).
Klein TM, Wolf ED, Wu R, Sanford J: High-velocity microprojectiles for delivering nucleic acids onto living cells. Nature 327: 70–73 (1987).
Kuntz M, Römer S, Suire C, Hugueney P, Weil JH, Schantz R, Camara B: Identification of a cDNA for the plastid-located geranylgeranyl pyrophosphate synthase from Capsicum annuum: correlative increase in enzyme activity and transcript level during fruit ripening. Plant J 2: 25–34 (1992).
Mann V, Pecker I, Hirschberg J: Cloning and characterization of the gene for phytoene desaturase (pds) from tomato (Lycopersicon esculentum). Plant Mol Biol 24: 429–434 (1994).
Ray J, Moureau P, Bird C, Bird A, Grierson D, Maunders M, Truesdale M, Bramley P, Schuch W: Cloning and characterization of a gene involved in phytoene synthesis from tomato. Plant Mol Biol 19: 401–404 (1992).
Römer S, Hugueney P, Bouvier F, Camara B, Kuntz M: Expression of the genes encoding the early carotenoid biosynthetic enzymes in Capsicum annuum. Biochem Biophys Res Commun 196: 1414–1421 (1993).
Sandmann G: Carotenoid biosynthesis in microorganisms and plants. Eur J Biochem 223: 7–24 (1994).
Scolnik PA, Bartley GE. Nucleotide sequence of an Arabidopsis cDNA for geranylgeranyl pyrophosphate synthase. Plant Physiol 104: 1469–1470 (1994).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Badillo, A., Steppuhn, J., Deruère, J. et al. Structure of a functional geranylgeranyl pyrophosphate synthase gene from Capsicum annuum . Plant Mol Biol 27, 425–428 (1995). https://doi.org/10.1007/BF00020196
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00020196