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Post-transcriptional regulation of a salt-inducible alfalfa gene encoding a putative chimeric proline-rich cell wall protein

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Abstract

A cDNA previously shown to identify a salt-inducible root-specific transcript in Medicago sativa was used to screen an alfalfa library for the corresponding genomic sequence. One positive clone was recovered. The nucleotide sequence of a subclone contained a 329 bp 5′ region upstream of the first ATG codon, a 1143 bp coding segment, and a 447 bp 3′-untranslated region interrupted by a single 475 bp intron. Translation of the coding segment, which was designated MsPRP2, suggested it encodes a chimeric 40 569 Da cell wall protein with an amino-terminal signal sequence, a repetitive proline-rich sequence, and a cysteine-rich carboxyl-terminal sequence homologous to nonspecific lipid transfer proteins. The 3′-untranslated region of MsPRP2 contained a sequence similar to one found to destabilize mRNAs transcribed from the elicitor-regulated proline-rich protein gene PvPRP1. Transcription run-on experiments using nuclei from salt-sensitive and salt-tolerant alfalfa callus suggested that the accumulation of MsPRP2 transcripts in salt-tolerant alfalfa cells grown in the presence of salt is due primarly to increased mRNA stability. The MsPRP2 gene thus may be a useful model for studying post-transcriptional salt-regulated expression of cell wall proteins.

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Author notes

  1. Charles E. Deutch

    Present address: Department of Biological Sciences, University of Nevada, 89154-4004, Las Vegas, NV, USA

Authors and Affiliations

  1. Department of Biochemistry, University of Nevada, 89557, Reno, NV, USA

    Charles E. Deutch & Ilga Winicov

  2. Department of Microbiology, University of Nevada, 89557, Reno, NV, USA

    Charles E. Deutch & Ilga Winicov

Authors
  1. Charles E. Deutch
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  2. Ilga Winicov
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Deutch, C.E., Winicov, I. Post-transcriptional regulation of a salt-inducible alfalfa gene encoding a putative chimeric proline-rich cell wall protein. Plant Mol Biol 27, 411–418 (1995). https://doi.org/10.1007/BF00020194

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  • DOI: https://doi.org/10.1007/BF00020194

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