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Sequence of two genes in pea chloroplast DNA coding for 84 and 82 kD polypeptides of the photosystem I complex

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Summary

The genes encoding the two P700 chlorophyll a-apoproteins of the photosystem I complex were localized on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the genes and the flanking regions has been determined. The genes are separated by 25 bp and are probably cotranscribed. The 5′ terminal gene (psaA1) codes for a 761-residue protein (MW 84.1 kD) and the 3′ terminal gene (psaA2) for a 734-residue protein (MW 82.4 kD). Both proteins are highly hydrophobic and contain eleven putative membrane-spanning domains. The homology to the corresponding polypeptides from maize are 89% and 95% for psaA1 and psaA2, respectively. A putative promoter has been identified for the psaA1 gene, and potential ribosome binding sites are present before both genes.

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Author notes

  1. O. F. Rasmussen

    Present address: Gensplejsningsgruppen, The Technical University of Denmark, building 227, 2800, Lyngby, Denmark

  2. G. B. Bookjans

    Present address: Albert Chandler Medical Center, Department of Biochemistry, University of Kentucky, Lexington, KY, USA

Authors and Affiliations

  1. Department of Genetics, The Royal Veterinary and Agricultural University, Bülowsvej 13, 1870, Copenhagen V, Denmark

    J. Lehmbeck, O. F. Rasmussen, G. B. Bookjans, B. R. Jepsen, B. M. Stummann & K. W. Henningsen

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  1. J. Lehmbeck
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  2. O. F. Rasmussen
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  3. G. B. Bookjans
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  4. B. R. Jepsen
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  5. B. M. Stummann
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  6. K. W. Henningsen
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Lehmbeck, J., Rasmussen, O.F., Bookjans, G.B. et al. Sequence of two genes in pea chloroplast DNA coding for 84 and 82 kD polypeptides of the photosystem I complex. Plant Mol Biol 7, 3–10 (1986). https://doi.org/10.1007/BF00020126

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  • DOI: https://doi.org/10.1007/BF00020126

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