Abstract
We have cloned and sequenced the cDNA encoding the open reading frame of the mRNA of the second largest subunit of RNA polymerase II, or RPB2, of tomato. The mRNA is transcribed from a single-copy gene in the tomato genome and the transcript size of the gene was measured as 4.2 kb by northern analysis. From the deduced amino acid sequence of 1191 residues, a protein of M r 135 000 with an isoelectric point of pH 7.9 was predicted. Alignment of the tomato RPB2 protein sequence with those of the homologous subunits in Arabidopsis, man, Drosophila and yeast showed considerable sequence identity.
References
Acker J, Wintzerith M, Vigneron M, Kedinger C: Primary structure of the second largest subunit of human RNA polymerase II (or B). J Mol Biol 226: 1295–1299 (1992).
la Cour TFM, Nyborg J, Thirup S, Clark BFC: Structural details of the binding of guanosine diphosphate to elongation factor Tu from E. coli as studied by X-ray. EMBO J 4: 2385–2388 (1985).
Devereux J: The GCG sequence analysis software package, Version 8. Genetics Computer Group (GCG), University Research Park, Madison, WI 53711 (1994).
Falkenburg D, Dworniczak B, Faust DM, Bautz KF: RNA polymerase II of Drosophila: relation of its 140 000 M r subunit to the β subunit of Escherichia coli RNA polymerase. J Mol Biol 195: 929–937 (1987).
Frohman MA: Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: thermal RACE. In: Methods Enzymol vol 218 pp 340–356 (1993).
Grachev MA, Hartmann GR, Maximova TG, Mustaec AA, Schäffner AR, Sieber H, Zaychikov EF: Highly selective affinity labelling of RNA polymerase B (II) from wheat germ. FEBS Lett 200: 287–290 (1986).
Guidet F, Rogowsky P, Taylor C, Song W, Langridge P: Cloning and characterisation of a new rye-specific repeated sequence. Genome 34: 81–87 (1990).
Kawagashi M, Yamagishi M, Ishihama A: Cloning and sequence determination of the Schizosachcaromyces pombe rpb2 gene encoding the subunit 2 of RNA polymerase II. Nucl Acids Res 21: 469–473 (1993).
Kozak M: Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiol Rev 47: 1–45 (1983).
Larkin R, Guilfoyle T: The second largest subunit of RNA polymerase II from Arabidopsis thaliana. Nucl Acids Res 21: 1038 (1993).
Maes M, Messens E: Phenol as a grinding material in RNA preparations. Nucl Acids Res 20: 4374 (1992).
Mustaev A, Kashlev M, Lee J, Polyakov A, Lebedev A, Zalenskaya K, Grachev, Nikiforov V: Mapping of the priming substrate contacts in the active center of Escherichia coli RNA polymerase. J Biol Chem 266: 23927–23931 (1991).
Ovchinnikov YA, Monastyrskaya GS, Gubanov VV, Guryev SO, Chertov OY, Modyanov NN, Grinkevich VA, Makarova IA, Marchenko TV, Polovnikova IN, Lipkin VM, Sverdlov ED: The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino acid sequence of the β subunit. Eur J Biochem 116: 621–629 (1981).
Riva M, Carles C, Sentenac A, Grachev MA, Arkady A, Mustaev A, Zaychikov EF: Mapping the active site of yeast RNA polymerase B(II). J Biol Chem 265: 16498–16503 (1990).
Sawadogo M, Sentenac A: RNA polymerase B (II) and general transcription factors. Annu Rev Biochem 59: 711–754 (1990).
Sitzler S, Oldenburg I, Petersen G, Bautz EKF: Analysis of the promoter of the housekeeping gene DmRP140 by sequence comparison of Drosophila melanogaster and Drosophila virilis. Gene 100: 155–162 (1991).
Sweetser D, Nonet M, Young RA: Prokaryotic and eukaryotic RNA polymerases have homologous core subunits. Proc Natl Acad Sci USA 84: 1192–1196 (1987).
Treich I, Riva M, Sentenac A: Zinc-binding subunits of yeast RNA polymerases. J Biol Chem 266: 21971–21976 (1991).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Warrilow, D., Symons, R.H. Sequence analysis of the second largest subunit of tomato RNA polymerase II. Plant Mol Biol 30, 337–342 (1996). https://doi.org/10.1007/BF00020119
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00020119