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Plant transformation: a simple particle bombardment device based on flowing helium

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Abstract

We observed that flowing helium at moderate pressures accelerated DNA-coated microprojectiles to velocities suitable for penetration of cells in intact plant tissues. The flowing helium principle permitted the construction of a simple and inexpensive transformation device that was easier to use than those previously described. This device provided efficient transformation of cells in soybean seedlings and other plants.

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Authors and Affiliations

  1. Department of Plant Pathology, University of California, 92521, Riverside, CA, USA

    Y. Takeuchi, M. Dotson & N. T. Keen

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  1. Y. Takeuchi
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  2. M. Dotson
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  3. N. T. Keen
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Takeuchi, Y., Dotson, M. & Keen, N.T. Plant transformation: a simple particle bombardment device based on flowing helium. Plant Mol Biol 18, 835–839 (1992). https://doi.org/10.1007/BF00020031

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  • DOI: https://doi.org/10.1007/BF00020031

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