Abstract
A cDNA encoding farnesyl diphosphate synthase, an enzyme that synthesizes C15 isoprenoid diphosphate from isopentenyl diphosphate and dimethylallyl diphosphate, was cloned from an Arabidopsis thaliana cDNA library by complementation of a mutant of Saccharomyces cerevisiae deficient in this enzyme. The A. thaliana cDNA was also able to complement the lethal phenotype of the erg20 deletion yeast mutant. As deduced from the full-length 1.22 kb cDNA nucleotide sequence, the polypeptide contains 343 amino acids and has a relative molecular mass of 39689. The predicted amino acid sequence presents about 50% identity with the yeast, rat and human FPP synthases. Southern blot analyses indicate that A. thaliana probably contains a single gene for farnesyl diphosphate synthase.
Similar content being viewed by others
References
Anderson MS, Yarger JG, Burck CL, Poulter CD: Farnesyl diphosphate synthetase: molecular cloning, sequence and expression of a essential gene from Saccharomyces cerevisiae. J Biol Chem 264: 19176–19184 (1989).
Ashby MN, Edwards PA: Elucidation of a deficiency in two yeast coenzyme Q mutants: Characterization of the structural gene encoding hexaprenyl pyrophosphate synthetase. J Biol Chem 265: 13157–13164 (1990).
Blanchard L, Karst F: Characterization of a lysine-to-glutamic acid mutation in a conservative sequence of farnesyl diphosphate synthase from Saccharomyces cerevisiae. Gene 125: 185–189 (1993).
Chambon C, Ladeveze V, Oulmouden A, Servouze M, Karst F: Isolation and properties of yeast mutants affected in farnesyl diphosphate synthetase. Curr Genet 18: 41–46 (1990).
Clarke CF, Tanaka RD, Svenson K, Wamsley M, Fogelman AM, Edwards PA: Molecular cloning and sequence of a cholesterol repressible enzyme related to prenyl transferase in the isoprene biosynthetic pathway. Mol Cell Biol 7: 3138–3146 (1987).
Cullin C, Pompon D: Synthesis of functional mouse cytochromes P-450 P1 and chimeric P-450 P3–1 in the yeast Saccharomyces cerevisiae. Gene 65: 203–217 (1988).
Elmberger PG, Kalen A, Appelkvist EL, Dallner G: In-vitro and in vivo synthesis of dolichol and other main mevalonate products in various organs of the rat. Eur J Biochem 168: 1–11 (1987).
Gietz RD, St Jean A, Woods RA, Schiestl RH: Improved method for high efficiency transformation of intact yeast cells. Nucl Acids Res 20: 1425 (1992).
Goodwin TW: The biochemistry of carotenoids. In: Plants, vol 1. Chapman and Hall, New York (1980).
Hancock JF, Magee AI, Childs JE, Marshall CJ: All ras proteins are polyisoprenylated but only some are palmitoylated. Cell 57: 1167–1177 (1989).
Kleinig H: The role of plastids in isoprenoid biosynthesis. Annu Rev Plant Physiol Plant Mol Biol 40: 39–59 (1989).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, NY (1982).
Math SK, Hearst JE, Poulter CD: The ctrE in Erwinia herbicola encodes geranylgeranyl diphosphate synthase. Proc Natl Acad Sci USA 89: 6761–6764 (1992).
Minet M, Dufour ME, Lacroute F: Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs. Plant J 2: 417–422 (1992).
Olson RE, Rudney H: Biosynthesis of ubiquinone. Vitam Horm 40: 1–43 (1983).
Poulter CD, Rilling HC: Prenyl transferases and isomerase. In: Porter JW, Spurgeon SL (eds) Biosynthesis of Isoprenoid Compounds, pp. 161–282. John Willey, New York (1981).
Rogers SO, Bendich AJ: Extraction of milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5: 69–76 (1985).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Servouze M, Karst F: Regulation of early enzymes of ergosterol biosynthesis in Saccharomyces cerevisae. Biochem J 240: 541–547 (1986).
Soler E, Clastre M, Bantignies B, Marigo G, Ambid C: Uptake of isopentenyl diphosphate into plastids isolated from Vitis vinifera L. suspensions. Planta 191: 324–329 (1993).
Wilkin DJ, Kutsunai SY, Edwards PA: Isolation and sequence of the human farnesyl pyrophosphate synthetase cDNA. J Biol Chem 265: 4607–4614 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Delourme, D., Lacroute, F. & Karst, F. Cloning of an Arabidopsis thaliana cDNA coding for farnesyl diphosphate synthase by functional complementation in yeast. Plant Mol Biol 26, 1867–1873 (1994). https://doi.org/10.1007/BF00019499
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00019499