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Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype

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Abstract

We have studied two lines of sunflower (Helianthus annuus L.) selected in the field as drought-tolerant (R1 genotype) or drought-sensitive (S1 genotype). When subjected to drought conditions, the R1 line was able to maintain high leaf water potential longer and wilted later than the S1 line. Therefore, this indicates that R1 tolerance includes a leafadaptive response. By subtractive hybridization, we have isolated six different cDNAs (designated sdi for sunflower drought-induced) corresponding to transcripts accumulated in R1 and S1 leaves during adaptive response. Analysis of transcript accumulation in response to drought in both genotypes suggests a preferential expression of three sdi genes in the tolerant line. Abscisic acid-mediated induction, analysed in R1 leaves, was observed for only four sdi genes. Sequence analysis of six sdi clones revealed that five clones were related to known proteins including non specific lipid transfer proteins (nsLTP), early light-induced proteins (ELIP), 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase) or dehydrins, predicted to be involved in a wide range of physiological processes.

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Authors and Affiliations

  1. Biochimie et Physiologie Végétales, UMII-CNRS URA 573-INRA-ENSAM, place Viala, 34060, Montpellier, Cedex 1, France

    Olivier Ouvrard, Françoise Cellier, Karine Ferrare, Didier Tousch, Thierry Lamaze & Francine Casse-Delbart

  2. Rhône-Poulenc Agrochimie, rue Pierre Baizet, 69009, Lyon, France

    Jean-Marc Dupuis

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  1. Olivier Ouvrard
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  2. Françoise Cellier
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Ouvrard, O., Cellier, F., Ferrare, K. et al. Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype. Plant Mol Biol 31, 819–829 (1996). https://doi.org/10.1007/BF00019469

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  • DOI: https://doi.org/10.1007/BF00019469

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