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Transcriptional regulation of a patatin-1 gene in potato

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Abstract

Patatin is an abundant glycoprotein in the tubers of potato plants that has a lipid acyl hydrolase activity. Fusions of the promoter of patatin genes that are highly expressed in tubers with the reporter gene encoding β-glucuronidase (GUS) have shown that patatin transcription has a high degree of tuber specificity. Patatin transcription was also inducible in other organs of transgenic potato by growth on high concentrations of sucrose. Experiments were conducted to define regions of the patatin promoter that confered tuber specific expression and sucrose inducibility. Sequences between -40 and -400 bp and between -400 and -957 bp of the transcriptional start site were able to confer tuber-specific expression on a heterologous truncated promoter. The cell specificity of GUS transcription in the transformants indicated that organ specificity was possibly determined by source-sink relationships of sucrose, or a metabolite of sucrose, in the whole plant.

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References

  1. Ahmed CMS, Sager GR: Patterns of distribution of assimilates in tubers ofSolanum tuberosum with time. Potato Res 24: 198–190 (1981).

    Google Scholar 

  2. Andrews DL, Beames B, Summers MD, Park WD: Characterisation of the lipid acyl hydrolase activity of the major potato tuber protein, patatin, by cloning and abundant expression in a baculovirus vector. Biochem J 252: 199–206 (1988).

    Google Scholar 

  3. Batutis EJ, Ewing EE: Far red reversal of red light effect during long night induction of potato. Plant Physiol 69: 672–674 (1982).

    Google Scholar 

  4. Benfey PN, Ren L, Chua N-H: The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. EMBO J 8: 2195–2202 (1989).

    Google Scholar 

  5. Bevan MW: BinaryAgrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).

    Google Scholar 

  6. Bevan MW, Barker R, Goldsbrough A, Jarvis M, Kavanagh T, Iturriaga G: The structure and transcription start site of a major potato tuber protein gene. Nucl Acids Res 14: 4625–4638 (1986).

    Google Scholar 

  7. Bradford M: A rapid and sensitive method for the quantitation of microgram amounts of protein using the principle of dye binding. Anal Biochem 72: 248–252 (1976).

    Google Scholar 

  8. Chen EY, Seeburg PH: Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4: 165–170 (1985).

    Google Scholar 

  9. Cutter EG: Structure and development of the potato plant. In: Harris PM (ed) The Potato Crop: The Scientific Basis for Improvement, pp. 70–152. Chapman and Hall, New York (1982).

    Google Scholar 

  10. Ewing EE, Waring PF: Shoot, stolon, and tuber formation on potato (Solanum tuberosum) cuttings in response to photoperiods. Plant Physiol 61: 348–353 (1978).

    Google Scholar 

  11. Ewing EE: Cuttings as simplified models of the potato plant. In Li PH (ed) Potato Physiology pp. 153–207. Academic Press, London (1985).

    Google Scholar 

  12. Forsline PL, Langille AR: An assessment of the modifying effect of kinetin on in vitro tuberisation of induced and non-induced tissues ofSolanum tuberosum. Can J Bot 54: 2513–2516 (1975).

    Google Scholar 

  13. Henikoff S: Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28: 351–359 (1984).

    Google Scholar 

  14. Humphreys TE: Phloem transport—with emphasis on loading and unloading. In: Baker DA, Hall JA (eds) Solute Transport in Plant Cells and Tissues, pp. 305–345. Longman Scientific, London (1988).

    Google Scholar 

  15. Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).

    Google Scholar 

  16. Kraus A: Interactions of nitrogen nutrition, phytohormones, and tuberisation. In: Li PH (ed) Potato Physiology, pp. 209–224. Academic Press, Lonson (1985).

    Google Scholar 

  17. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1982).

    Google Scholar 

  18. Mignery CA, Pikaard CS, Park WD: Molecular characterisation of the patatin multigene family of potato. Gene 62: 27–44 (1988).

    Google Scholar 

  19. Murashige J, Skoog F: A revised medium for rapid growth and bioassays with tobacco tissue cultivars. Physiol Plant 15: 473–497 (1962).

    Google Scholar 

  20. Odell JT, Knowlton S, Lin W, Mauvais CJ: Properties of an isolated stimulating sequence from the cauliflower mosaic virus 35S promoter. Plant Mol Biol 10: 263–272 (1988).

    Google Scholar 

  21. Oparka KJ, Prior DAH: Movement of Lucifer Yellow CH in potato tuber storage tissue: a comparison of symplastic and apoplastic transport. Planta 176: 533–540 (1988).

    Google Scholar 

  22. Paiva E, Lister PM, Park WD: Induction and accumulation of major tuber proteins of potato in stems and petioles. Plant Physol 71: 616–618 (1983).

    Google Scholar 

  23. Peterson RL, Barker GW, Howarth MJ: Development and structure of tubers. In: Li PH (ed) Potato Physiology, pp. 124–148. Academic Press, London (1985).

    Google Scholar 

  24. Pikaard CS, Brusca JS, Hannapel DJ, Park WD: The two classes of genes for the major potato tuber protein, patatin, are differentially expressed in tubers and roots. Nucl Acids Res 15: 1979–1994 (1987).

    Google Scholar 

  25. Racusen D: Occurence of patatin during growth and storage of potato tubers. Can J Bot 61: 370–373 (1983).

    Google Scholar 

  26. Racusen D: Esterase specificity of patatin from two potato cultivars. Can J Bot 64: 2104–2106 (1985).

    Google Scholar 

  27. Rocha-Sosa M, Sonnewald U, Frommer W, Stratmann M, Schell J, Willmitzer L: Both developmental and metabolic signals activate the promoter of a class I patatin gene. EMBO J 8: 23–31 (1989).

    Google Scholar 

  28. Rosahl S, Schmidt R, Schell J, Willmitzer L: Isolation and characterisation of a gene fromSolanum tuberosum encoding patatin, the major storage protein of potato tubers. Mol Gen Genet 203: 214–220 (1986).

    Google Scholar 

  29. Rosahl S, Schell J, Willmitzer L: Expression of a tuber specific storage protein in transgenic tobacco plants: demonstration of an esterase activity. EMBO J 6: 115–119 (1987).

    Google Scholar 

  30. Sheerman S, Bevan MW: A rapid transformation method forSolanum tuberosum using binaryAgrobacterium tumefaciens vectors. Plant Cell Rep 7: 13–16 (1988).

    Google Scholar 

  31. Staswick PE: Developmental regulation and influence of plant sinks on vegetative storage protein gene expression in soybean leaves. Plant Physol 89: 309–315 (1988).

    Google Scholar 

  32. Twell D, Ooms G: The 5′ flanking region of a patatin gene directs tuber specific expression of a chimaeric gene in potato. Plant Mol Bol 9: 365–375 (1987).

    Google Scholar 

  33. Twell D, Ooms G: Structural diversity of the patatin multigene family in potato cv. Desiree. Mol Gen Genet 212: 325–336 (1988).

    Google Scholar 

  34. Wenzler HC, Mignery GA, Fisher LM, Park WD: Analysis of a chimaeric class I patatin-GUS gene fusion in transgenic potato plants: high level expression in tubers and sucrose-inducible expression in cultured leaf and stem explants. Plant Mol Biol 12: 41–50 (1989).

    Google Scholar 

  35. Wright KM, Oparka KJ: Sucrose uptake and partitioning in discs derived from source versus sink potato tubers. Planta 177: 237–244 (1989).

    Google Scholar 

  36. Zimmermann MH, Ziegler H: List of sugars and sugar alcohols in sieve-tube exudates. In: Zimmermann MH, Milburn JA (eds) Transport in Plants, Encyclopaedia of Plant Physiology, vol. 1, pp. 480–503. Springer-Verlag, Heidelberg (1975).

    Google Scholar 

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Authors and Affiliations

  1. Institute of Plant Science Research, Cambridge Laboratory, Colney Lane, NR4 74J, Norwich, England

    Richard Jefferson, Andrew Goldsbrough & Michael Bevan

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  1. Richard Jefferson
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  2. Andrew Goldsbrough
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  3. Michael Bevan
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Jefferson, R., Goldsbrough, A. & Bevan, M. Transcriptional regulation of a patatin-1 gene in potato. Plant Mol Biol 14, 995–1006 (1990). https://doi.org/10.1007/BF00019396

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  • DOI: https://doi.org/10.1007/BF00019396

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